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Constructing Synthetic Microbial Communities to Explore the Ecology and Evolution of Symbiosis

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Engineering and Analyzing Multicellular Systems

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1151))

Abstract

Synthetically engineered microbial communities based on model organisms provide a simplified model of their naturally occurring counterparts while still retaining essential features of living organisms. The degree of control afforded by this approach has been critical in understanding how similar types of natural communities might have persisted and evolved. Here, we first discuss important considerations when designing a synthetically engineered system. Then, we describe the steps required to create a two-partner cooperative system based on the yeast Saccharomyces cerevisiae.

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Acknowledgements

Work in the W.S. group is supported by the W. M. Keck Foundation and the National Institutes of Health (Grant 1 DP2OD006498-01).

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Authors and Affiliations

  1. Molecular and Cellular Biology Program, University of Washington, Seattle, WA, 98185, USA

    Adam James Waite

  2. Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA, 98109, USA

    Adam James Waite & Wenying Shou

Authors
  1. Adam James Waite
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  2. Wenying Shou
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Corresponding author

Correspondence to Wenying Shou .

Editor information

Editors and Affiliations

  1. School of Life Sciences, University of Science & Technology of China,, Hefei, Anhui, China

    Lianhong Sun

  2. Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA

    Wenying Shou

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Waite, A.J., Shou, W. (2014). Constructing Synthetic Microbial Communities to Explore the Ecology and Evolution of Symbiosis. In: Sun, L., Shou, W. (eds) Engineering and Analyzing Multicellular Systems. Methods in Molecular Biology, vol 1151. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0554-6_2

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  • DOI: https://doi.org/10.1007/978-1-4939-0554-6_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0553-9

  • Online ISBN: 978-1-4939-0554-6

  • eBook Packages: Springer Protocols

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