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A Highly Reproducible Mouse Model of Compression Spinal Cord Injury

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Axon Growth and Regeneration

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1162))

Abstract

Experimental spinal cord injury (SCI) can maintain the continuity of the spinal cord, as in the contusion (e.g., weight-fall) or compression models, or not, when there is a partial or a complete transection. The majority of acute human SCI is not followed by complete transection, but there is a combination of contusion, compression, and possibly partial transection. The method described here is a compressive mouse model that presents a combination of contusion and compression components and has many facilities in its execution. This lesion was established by our group and represents a simple, reliable, and inexpensive clip compression model with functional and morphological reproducibility. In this chapter we describe, step by step, the protocol of this experimental SCI.

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Author information

Authors and Affiliations

  1. Laboratório de Regeneração Neural e Função, Departamento de Neurobiologia, Fluminense Federal University, Niterói, RJ, Brazil

    Suelen Adriani Marques

  2. Laboratório de Neurodegeneração e Reparo, Centro de Ciências da Saúde, Federal University of Rio de Janeiro, Av. Rodolfo Paulo Rocco, 255, F2-12, Rio de Janeiro, RJ, Brazil

    Ana Maria Blanco Martinez M.D., Ph.D.

  3. Instituto de Ciências Biomédicas, Cidade Universitária, Ilha do Fundão, 21941-913, Rio de Janeiro, RJ, Brazil

    Ana Maria Blanco Martinez M.D., Ph.D.

  4. Universidade Federal do Rio de Janeiro Campus Macaé, Av. Aluízio da Silva Gomes 50, Granja dos Cavaleiros, Macaé, RJ, Brazil

    Fernanda Martins de Almeida

  5. Instituto de Ciências Biomédicas, Centro de Ciências da Saúde, F2-12, Federal University of Rio de Janeiro, Av. Rodolfo Paulo Rocco, 255, 21941-913, Rio de Janeiro, RJ, Brazil

    Klauss Mostacada

Authors
  1. Suelen Adriani Marques
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  2. Fernanda Martins de Almeida
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  3. Klauss Mostacada
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  4. Ana Maria Blanco Martinez M.D., Ph.D.
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Corresponding author

Correspondence to Ana Maria Blanco Martinez M.D., Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York, USA

    Andrew J. Murray

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Marques, S.A., de Almeida, F.M., Mostacada, K., Martinez, A.M.B. (2014). A Highly Reproducible Mouse Model of Compression Spinal Cord Injury. In: Murray, A. (eds) Axon Growth and Regeneration. Methods in Molecular Biology, vol 1162. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0777-9_12

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  • DOI: https://doi.org/10.1007/978-1-4939-0777-9_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0776-2

  • Online ISBN: 978-1-4939-0777-9

  • eBook Packages: Springer Protocols

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