Abstract
Mimotopes are peptides mimicking epitopes on the corresponding antigen. They can be obtained via panning the phage-displayed random peptide library against the corresponding monoclonal antibody or specific sera. Besides mimotopes however, the experimental results also include all kinds of unwanted sequences called “target-unrelated peptides,” which often interfere with the subsequent experimental and computational analyses. Nevertheless, the prediction of B-cell epitopes based on the experimental result of phage display has shown to be a promising and reliable strategy with acceptable precision. In this chapter, we summarize mimotope-based prediction of B-cell epitopes under three conditions and focus on protocols and tips for retrieving, cleaning, and decoding the data from phage display technology.
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Acknowledgments
This chapter was supported in part by the National Natural Science Foundation of China under Grant 61071177 and the Program for New Century Excellent Talents in University (NCET-12-0088).
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Huang, J., He, B., Zhou, P. (2014). Mimotope-Based Prediction of B-Cell Epitopes. In: De, R., Tomar, N. (eds) Immunoinformatics. Methods in Molecular Biology, vol 1184. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1115-8_13
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DOI: https://doi.org/10.1007/978-1-4939-1115-8_13
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