Skip to main content
SpringerLink
Log in
Book cover

Tissue Morphogenesis

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Celeste M. Nelson

    1. Chemical & Biological Engineering, Princeton University, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1189)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (22 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Imaging Morphogenesis

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Probing Regional Mechanical Properties of Embryonic Tissue Using Microindentation and Optical Coherence Tomography

      • Benjamen A. Filas, Gang Xu, Larry A. Taber
      Pages 3-16

    3. Hemodynamic Flow Visualization of Early Embryonic Great Vessels Using μPIV

      • Selda Goktas, Chia-Yuan Chen, William J. Kowalski, Kerem Pekkan
      Pages 17-30

    4. Using Correlative Light and Electron Microscopy to Study Zebrafish Vascular Morphogenesis

      • Jacky G. Goetz, Fabien Monduc, Yannick Schwab, Julien Vermot
      Pages 31-46

    5. Micro/Nano-Computed Tomography Technology for Quantitative Dynamic, Multi-scale Imaging of Morphogenesis

      • Chelsea L. Gregg, Andrew K. Recknagel, Jonathan T. Butcher
      Pages 47-61

    6. Imaging the Dorsal-Ventral Axis of Live and Fixed Drosophila melanogaster Embryos

      • Sophia N. Carrell, Gregory T. Reeves
      Pages 63-78

    7. Light Sheet-Based Imaging and Analysis of Early Embryogenesis in the Fruit Fly

      • Khaled Khairy, William C. Lemon, Fernando Amat, Philipp J. Keller
      Pages 79-97

    8. Quantitative Image Analysis of Cell Behavior and Molecular Dynamics During Tissue Morphogenesis

      • Chun Yin Bosco Leung, Rodrigo Fernandez-Gonzalez
      Pages 99-113

    9. A Multiplex Fluorescent In Situ Hybridization Protocol for Clonal Analysis of Drosophila Oogenesis

      • Lily S. Cheung, Stanislav Shvartsman
      Pages 115-122

    10. Active Cell and ECM Movements During Development

      • Anastasiia Aleksandrova, Brenda J. Rongish, Charles D. Little, András Czirók
      Pages 123-132

  3. Culture Models of Morphogenesis

    1. Front Matter

      Pages 133-133
      Download chapter PDF

    2. 3D Culture Assays of Murine Mammary Branching Morphogenesis and Epithelial Invasion

      • Kim-Vy Nguyen-Ngoc, Eliah R. Shamir, Robert J. Huebner, Jennifer N. Beck, Kevin J. Cheung, Andrew J. Ewald
      Pages 135-162

    3. Culture of Mouse Embryonic Foregut Explants

      • Felicia Chen, Wellington V. Cardoso
      Pages 163-169

    4. Investigating Human Vascular Tube Morphogenesis and Maturation Using Endothelial Cell-Pericyte Co-cultures and a Doxycycline-Inducible Genetic System in 3D Extracellular Matrices

      • Stephanie L. K. Bowers, Chun-Xia Meng, Matthew T. Davis, George E. Davis
      Pages 171-189

    5. Three-Dimensional Traction Force Microscopy of Engineered Epithelial Tissues

      • Alexandra S. Piotrowski, Victor D. Varner, Nikolce Gjorevski, Celeste M. Nelson
      Pages 191-206

  4. Manipulating Cells and Tissues In Vivo

    1. Front Matter

      Pages 207-207
      Download chapter PDF

    2. Probing Cell Mechanics with Subcellular Laser Dissection of Actomyosin Networks in the Early Developing Drosophila Embryo

      • M. Rauzi, P.-F. Lenne
      Pages 209-218

    3. UV Laser Ablation to Measure Cell and Tissue-Generated Forces in the Zebrafish Embryo In Vivo and Ex Vivo

      • Michael Smutny, Martin Behrndt, Pedro Campinho, Verena Ruprecht, Carl-Philipp Heisenberg
      Pages 219-235

    4. Measurement of Intercellular Cohesion by Tissue Surface Tensiometry

      • Ramsey A. Foty
      Pages 237-254
Back to top

Keywords

About this book

Tissue Morphogenesis: Methods and Protocols highlights major techniques, both experimental and computational, for the study of tissue morphogenesis, divided into several sections, with specific focus on techniques to image, manipulate, model and analyze tissue morphogenesis. Chapters focus on imagining analysis of tissue morphogenesis, culture models of tissue morphogenesis, manipulating cells and tissues in vivo, novel model systems to investigate issue morphogenesis and computational models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Tissue Morphogenesis: Methods and Protocols serves as a primary resource for both fundamental and practical understanding of the techniques used to uncover the basis of tissue morphogenesis.

Editors and Affiliations

  • Chemical & Biological Engineering, Princeton University, USA

    Celeste M. Nelson

Bibliographic Information

  • Book Title: Tissue Morphogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Celeste M. Nelson

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1164-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-1163-9Published: 23 September 2014

  • Softcover ISBN: 978-1-4939-4585-6Published: 30 April 2017

  • eBook ISBN: 978-1-4939-1164-6Published: 22 September 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 351

  • Number of Illustrations: 30 b/w illustrations, 76 illustrations in colour

  • Topics: Cell Biology, Gene Function

Publish with us

Policies and ethics

Back to top

Search

Navigation