Abstract
Mechanically coupled cells can generate forces driving cell and tissue morphogenesis during development. Visualization and measuring of these forces is of major importance to better understand the complexity of the biomechanic processes that shape cells and tissues. Here, we describe how UV laser ablation can be utilized to quantitatively assess mechanical tension in different tissues of the developing zebrafish and in cultures of primary germ layer progenitor cells ex vivo.
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Acknowledgements
We are grateful to R. Hauschild for advice and assistance to experimental work and the service facilities of the IST Austria.
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Smutny, M., Behrndt, M., Campinho, P., Ruprecht, V., Heisenberg, CP. (2015). UV Laser Ablation to Measure Cell and Tissue-Generated Forces in the Zebrafish Embryo In Vivo and Ex Vivo. In: Nelson, C. (eds) Tissue Morphogenesis. Methods in Molecular Biology, vol 1189. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1164-6_15
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DOI: https://doi.org/10.1007/978-1-4939-1164-6_15
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1163-9
Online ISBN: 978-1-4939-1164-6
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