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Yeast Genetics pp 1–12Cite as

Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1205))

Abstract

Efficient transformation of yeast has been the corner stone of molecular studies in yeast for numerous years. It is essential for techniques such as the yeast two-hybrid system as well as genome modification. Here I present protocols for a number of different transformation applications. A quick and easy protocol is for situations in which large numbers of transformants are not required. The high-efficiency protocol generates large numbers of transformants from small amounts of DNA that can be scaled up for the library transformation protocol. A transformation protocol for a microtiter plate format is also included. Finally, a protocol for the production of frozen competent yeast cells is included, which allows cells to be taken from the freezer at a moment’s notice and transformed with high efficiency.

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References

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Medical Genetics, University of Manitoba, Room 336, Basic Medical Sciences Building, Bannatyne Campus, 745 Bannatyne Avenue, Winnipeg, MB, Canada, R3E 0J9

    R. Daniel Gietz

Authors
  1. R. Daniel Gietz
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Corresponding author

Correspondence to R. Daniel Gietz .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia, USA

    Jeffrey S. Smith

  2. Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia, USA

    Daniel J. Burke

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Gietz, R.D. (2014). Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method. In: Smith, J., Burke, D. (eds) Yeast Genetics. Methods in Molecular Biology, vol 1205. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1363-3_1

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  • DOI: https://doi.org/10.1007/978-1-4939-1363-3_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1362-6

  • Online ISBN: 978-1-4939-1363-3

  • eBook Packages: Springer Protocols

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