Abstract
RNA-centric biochemical purification is a general approach for studying the functions and mechanisms of noncoding RNAs. Here, we describe the experimental procedures for RNA antisense purification (RAP), a method for selective purification of endogenous RNA complexes from cell extracts that enables mapping of RNA interactions with chromatin. In RAP, the user cross-links cells to fix endogenous RNA complexes and purifies these complexes through hybrid capture with biotinylated antisense oligos. DNA loci that interact with the target RNA are identified using high-throughput DNA sequencing.
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Engreitz, J., Lander, E.S., Guttman, M. (2015). RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin. In: Nakagawa, S., Hirose, T. (eds) Nuclear Bodies and Noncoding RNAs. Methods in Molecular Biology, vol 1262. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2253-6_11
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DOI: https://doi.org/10.1007/978-1-4939-2253-6_11
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-2253-6
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