Abstract
Wild populations of the model organism C. elegans allow characterization of natural genetic variation underlying diverse phenotypic traits. Here we provide a simple protocol on how to sample and rapidly identify C. elegans wild isolates. We outline how to find suitable habitats and organic substrates, followed by describing isolation and identification of C. elegans live cultures based on easily recognizable morphological characteristics, molecular barcodes and/or mating tests. This protocol uses standard laboratory equipment and requires no prior knowledge of C. elegans biology.
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Acknowledgments
This protocol makes use of diverse contributions from the worm community and is primarily based on Caenorhabditis isolation methods established by Antoine Barrière and Marie-Anne Félix. Our research is financed by the Centre National de la Recherche Scientifique, France.
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Poullet, N., Braendle, C. (2015). Sampling and Isolation of C. elegans from the Natural Habitat. In: Biron, D., Haspel, G. (eds) C. elegans. Methods in Molecular Biology, vol 1327. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4939-2842-2_16
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DOI: https://doi.org/10.1007/978-1-4939-2842-2_16
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-4939-2841-5
Online ISBN: 978-1-4939-2842-2
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