Abstract
Recently we have explored and developed approaches imaging using confocal/two-photon microscopy, which enables simultaneous high-resolution assessment of specifically fluorescently marked cells in conjunction with structural components of the tissues visualized via harmonic generated signals. This approach uses commercially available confocal and two-photon laser microscope and automated user-interactive image analysis methods based on commercially available software packages allowing easy implementation in usual microscopy facilities.
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Acknowledgements
This work was supported by intramural funding of the Division of Intramural Research, National Heart, Lung, and Blood Institute, NIH. We thank Dr. Xingmin Feng (Hematology Branch, NHLBI) for providing the mice, Dr. Christian A. Combs for the overall support of NHLBI light microscopy core facility, and Brent Nettrour, Peter Pecoraro, for the technical support of the microscope.
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Malide, D. (2016). In Vivo Cell Tracking Using Two-Photon Microscopy. In: Bai, M. (eds) In Vivo Fluorescence Imaging. Methods in Molecular Biology, vol 1444. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3721-9_11
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DOI: https://doi.org/10.1007/978-1-4939-3721-9_11
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-3721-9
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