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Analysis of Meiotic Recombination in Caenorhabditis elegans

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Book cover Meiosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 557))

Abstract

Caenorhabditis elegans is an important experimental organism for the study of recombination during meiosis. A variety of techniques have been developed for the measurement of meiotic recombination in C. elegans, ranging from traditional genetic measures to direct cytological determination of chiasma frequency. Here, we provide methods for some of the varied approaches used for the study of meiotic recombination in these tiny but powerful worms.

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References

  1. Keeney, S., Giroux, C. N., and Kleckner, N. (1997) Meiosis-specific DNA double-strand breaks are catalyzed by Spo11, a member of a widely conserved protein family. Cell 88, 375–84.

    Article  PubMed  CAS  Google Scholar 

  2. Dernburg, A. F., McDonald, K., Moulder, G., Barstead, R., Dresser, M., and Villeneuve, A. M. (1998) Meiotic recombination in C. elegans initiates by a conserved mechanism and is dispensable for homologous chromosome synapsis. Cell 94, 387–98.

    Article  PubMed  CAS  Google Scholar 

  3. Kim, J. S., and Rose, A. M. (1987) The effect of gamma radiation on recombination frequency in Caenorhabditis elegans. Genome 29, 457–62.

    Article  PubMed  CAS  Google Scholar 

  4. Petronczki, M., Siomos, M. F., and Nasmyth, K. (2003) Un menage a quatre: the molecular biology of chromosome segregation in meiosis. Cell 112, 423–40.

    Article  PubMed  CAS  Google Scholar 

  5. Villeneuve, A. M. (1994) A cis-acting locus that promotes crossing over between X chromosomes in Caenorhabditis elegans. Genetics 136, 887–902.

    PubMed  CAS  Google Scholar 

  6. Stiernagle, T. (2006) WormBook, ed. the C. elegans Research Community, http://www.wormbook.org.

  7. Davis, M. W., and Hammarlund, M. (2006) Single-nucleotide polymorphism mapping. Methods Mol. Biol. 351, 75–92.

    PubMed  CAS  Google Scholar 

  8. Brenner, S. (1974) The genetics of Caenorhabditis elegans. Genetics 77, 71–94.

    PubMed  CAS  Google Scholar 

  9. Wicks, S. R., Yeh, R. T., Gish, W. R., Waterston, R. H., and Plasterk, R. H. (2001) Rapid gene mapping in Caenorhabditis elegans using a high density polymorphism map. Nat. Genet. 28, 160–4.

    Article  PubMed  CAS  Google Scholar 

  10. Hammarlund, M., Davis, M. W., Nguyen, H., Dayton, D., and Jorgensen, E. M. (2005) Heterozygous insertions alter crossover distribution but allow crossover interference in Caenorhabditis elegans. Genetics 171, 1047–56.

    Article  PubMed  CAS  Google Scholar 

  11. Nabeshima, K., Villeneuve, A. M., and Hillers, K. J. (2004) Chromosome-wide regulation of meiotic crossover formation in Caenorhabditis elegans requires properly assembled chromosome axes. Genetics 168, 1275–92.

    Article  PubMed  CAS  Google Scholar 

  12. Alpi, A., Pasierbek, P., Gartner, A., and Loidl, J. (2003) Genetic and cytological characterization of the recombination protein RAD-51 in Caenorhabditis elegans. Chromosoma 112, 6–16.

    Article  PubMed  CAS  Google Scholar 

  13. Martin, J. S., Winkelmann, N., Petalcorin, M. I., McIlwraith, M. J., and Boulton, S. J. (2005) RAD-51-dependent and -independent roles of a Caenorhabditis elegans BRCA2-related protein during DNA double-strand break repair. Mol. Cell. Biol. 25, 3127–39.

    Article  PubMed  CAS  Google Scholar 

  14. Rinaldo, C., Bazzicalupo, P., Ederle, S., Hilliard, M., and La Volpe, A. (2002) Roles for Caenorhabditis elegans rad-51 in meiosis and in resistance to ionizing radiation during development. Genetics 160, 471–9.

    PubMed  CAS  Google Scholar 

  15. Liu, H., Jang, J. K., Kato, N., and McKim, K. S. (2002) mei-P22 encodes a chromosome-associated protein required for the initiation of meiotic recombination in Drosophila melanogaster. Genetics 162, 245–58.

    PubMed  CAS  Google Scholar 

  16. Thorne, L. W., and Byers, B. (1993) Stage-specific effects of X-irradiation on yeast meiosis. Genetics 134, 29–42.

    PubMed  CAS  Google Scholar 

  17. Chin, G. M., and Villeneuve, A. M. (2001) C. elegans mre-11 is required for meiotic recombination and DNA repair but is dispensable for the meiotic G(2) DNA damage checkpoint. Genes Dev. 15, 522–34.

    Article  PubMed  CAS  Google Scholar 

  18. Colaiacovo, M. P., MacQueen, A. J., Martinez-Perez, E., McDonald, K., Adamo, A., La Volpe, A., and Villeneuve, A. M. (2003) Synaptonemal complex assembly in C. elegans is dispensable for loading strand-exchange proteins but critical for proper completion of recombination. Dev. Cell 5, 463–74.

    Article  PubMed  CAS  Google Scholar 

  19. Hayashi, M., Chin, G. M., and Villeneuve, A. M. (2007) C. elegans germ cells switch between distinct modes of double-strand break repair during meiotic prophase progression. PLoS Genet. 3, 2068–84.

    Article  CAS  Google Scholar 

  20. McKim, K. S., Howell, A. M., and Rose, A. M. (1988) The effects of translocations on recombination frequency in Caenorhabditis elegans. Genetics 120, 987–1001.

    PubMed  CAS  Google Scholar 

  21. Edgley, M. L., Baillie, D. L., Riddle, D. L., and Rose, A. M. (2006) Genetic balancers. WormBook, ed. the C. elegans Research Community, http://www.wormbook.org.

  22. Kelly, K. O., Dernburg, A. F., Stanfield, G. M., and Villeneuve, A. M. (2000) Caenorhabditis elegans msh-5 is required for both normal and radiation- induced meiotic crossing over but not for completion of meiosis. Genetics 156, 617–30.

    PubMed  CAS  Google Scholar 

  23. Zetka, M. C., and Rose, A. M. (1990) Sex-related differences in crossing over in Caenorhabditis elegans. Genetics 126, 355–63.

    PubMed  CAS  Google Scholar 

  24. Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., and Mello, C. C. (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391, 806–11.

    Article  PubMed  CAS  Google Scholar 

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Hillers, K.J., Villeneuve, A.M. (2009). Analysis of Meiotic Recombination in Caenorhabditis elegans . In: Keeney, S. (eds) Meiosis. Methods in Molecular Biology, vol 557. Humana Press. https://doi.org/10.1007/978-1-59745-527-5_7

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  • DOI: https://doi.org/10.1007/978-1-59745-527-5_7

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-934115-66-4

  • Online ISBN: 978-1-59745-527-5

  • eBook Packages: Springer Protocols

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