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Enumeration of Bacteriophages Using the Small Drop Plaque Assay System

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 501))

Abstract

The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. Described here is a drop plaque assay, which, being simpler, faster and more efficient than either the classical overlay or direct plating methods, enhances efficiency in processing large numbers of samples.

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References

  1. Arber, W. L., Enquist, L., Hohn, B., Murray, N. E., & Murray, K. (1983) in Lambda II (Cold Spring Harbor Laboratory Press, Cold Spring Harbor Laboratory,NY), p. 433.

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  2. Sambrook, J. & Russell, D. W. (2001) Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Press, Cold Spring Harbor, New York).

    Google Scholar 

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Mazzocco, A., Waddell, T.E., Lingohr, E., Johnson, R.P. (2009). Enumeration of Bacteriophages Using the Small Drop Plaque Assay System. In: Clokie, M.R., Kropinski, A.M. (eds) Bacteriophages. Methods in Molecular Biology™, vol 501. Humana Press. https://doi.org/10.1007/978-1-60327-164-6_9

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  • DOI: https://doi.org/10.1007/978-1-60327-164-6_9

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-682-5

  • Online ISBN: 978-1-60327-164-6

  • eBook Packages: Springer Protocols

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