Summary
Transgenesis is an important methodology for studying the function of genes and genomes in model plants and animals. For the model vertebrate zebrafish, methods using the Tol2 transposable element have been developed for this purpose. With these methods, the function of the transgene can be analyzed in both transient and stable transgenic fish. Recently, cis-sequences necessary for transposition of the Tol2 element were revealed. This enabled development of transposon vectors containing minimal DNA sequences that are easily manipulated. More recently, several transposon vectors containing the Gateway sequence were created and reported. These are useful because any foreign sequences can be cloned into a transposon vector fairly easily and rapidly. This chapter describes the features of these transposon vectors, and protocols to perform transgenesis in zebrafish.
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Acknowledgments
We would like to thank Dr. Chi-Bin Chien for helpful comments on the manuscript; a postdoctoral fellowship from National Institute of Genetics (to HK); and the National BioResource Project and grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
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Kikuta, H., Kawakami, K. (2009). Transient and Stable Transgenesis Using Tol2 Transposon Vectors. In: Lieschke, G., Oates, A., Kawakami, K. (eds) Zebrafish. Methods in Molecular Biology, vol 546. Humana Press. https://doi.org/10.1007/978-1-60327-977-2_5
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DOI: https://doi.org/10.1007/978-1-60327-977-2_5
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