Summary
When a cell migrates, it generates traction forces on the underlying substrate. The cell traction force (CTF) is not only essential for cell migration, but it is also used by cells to control their shape and maintain cellular homeostasis. As such, quantification of CTFs aids in better understanding of many fundamental biological processes such as morphogenesis, angiogenesis, and wound healing of tissues and organs. A new technology called cell traction force microscopy (CTFM) has been developed to determine CTFs in a quantitative fashion. The advantage of this technology is that it directly measures the “cause” (i.e., CTFs) of cell movement instead of the “effect” (i.e., cell movement itself), which is measured by various conventional methods. This chapter provides detailed information of materials and methods that are needed in order to perform typical CTFM experiments. Several examples are also given to illustrate various applications of CTFM in cell biology research.
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Rodriguez, L.G., Wu, X. and Guan, J.L. (2005) Wound-healing assay. Methods Mol Biol 294, 23–29.
Thampatty, B.P. and Wang, J.H. (2007) A new approach to study fibroblast migration. Cell Motil Cytoskeleton 64, 1–5.
Ananthakrishnan, R. and Ehrlicher, A. (2007) The forces behind cell movement. Int J Biol Sci 3, 303–317.
Lauffenburger, D.A. and Horwitz, A.F. (1996) Cell migration: a physically integrated molecular process. Cell 84, 359–369.
Oliver, T., Dembo, M. and Jacobson, K. (1995) Traction forces in locomoting cells. Cell Motil Cytoskeleton 31, 225–240.
Wang, J.H. and Lin, J.S. (2007) Cell traction force and measurement methods. Biomech Model Mechanobiol 6, 361–371.
Burton, K., Park, J.H. and Taylor, D.L. (1999) Keratocytes generate traction forces in two phases. Mol Biol Cell 10, 3745–3769.
Harris, A.K., Wild, P. and Stopak, D. (1980) Silicone rubber substrata: a new wrinkle in the study of cell locomotion. Science 208, 177–179.
Balaban, N.Q., Schwarz, U.S., Riveline, D., et al. (2001) Force and focal adhesion assembly: a close relationship studied using elastic micropatterned substrates. Nat Cell Biol 3, 466–472.
Galbraith, C.G. and Sheetz, M.P. (1997) A micromachined device provides a new bend on fibroblast traction forces. Proc Natl Acad Sci U S A 94, 9114–9118.
du Roure, O., Saez, A., Buguin, A., et al. (2005) Force mapping in epithelial cell migration. Proc Natl Acad Sci U S A 102, 2390–2395.
Tan, J.L., Tien, J., Pirone, D.M., et al. (2003) Cells lying on a bed of microneedles: an approach to isolate mechanical force. Proc Natl Acad Sci U S A 100, 1484–1489.
Li, B., Xie, L., Starr, Z.C., et al. (2007) Development of micropost force sensor array with culture experiments for determination of cell traction forces. Cell Motil Cytoskeleton 64, 509–518
Dembo, M. and Wang, Y.L. (1999) Stresses at the cell-to-substrate interface during locomotion of fibroblasts. Biophys J 76, 2307–2316.
Butler, J.P., Tolic-Norrelykke, I.M., Fabry, B., et al. (2002) Traction fields, moments, and strain energy that cells exert on their surroundings. Am J Physiol Cell Physiol 282, C595–C605.
Yang, Z., Lin, J.S., Chen, J., et al. (2006) Determining substrate displacement and cell traction fields – a new approach. J Theor Biol 242, 607–616.
Pelham, R.J., Jr. and Wang, Y. (1997) Cell locomotion and focal adhesions are regulated by substrate flexibility. Proc Natl Acad Sci U S A 94, 13661–13665.
Kandow, C.E., Georges, P.C., Janmey, P.A., et al. (2007) Polyacrylamide hydrogels for cell mechanics: steps toward optimization and alternative uses. Methods Cell Biol 83, 29–46.
Chen, J., Li, H., Sundarraj, N., et al. (2007) Alpha-smooth muscle actin expression enhances cell traction force. Cell Motil Cytoskeleton 64, 248–257.
Lo, C.M., Wang, H.B., Dembo, M., et al. (2000) Cell movement is guided by the rigidity of the substrate. Biophys J 79, 144–152.
Li, Y., Hu, Z. and Li, C. (1993) New method for measuring Poisson’s ratio in polymer gels. J Appl Polym Sci 50, 1107–1111.
Anderson, S., DiCesare, L., Tan, I., et al. (2004) Rho-mediated assembly of stress fibers is differentially regulated in corneal fibroblasts and myofibroblasts. Exp Cell Res 298, 574–583.
Hinz, B., Celetta, G., Tomasek, J.J., et al. (2001) Alpha-smooth muscle actin expression upregulates fibroblast contractile activity. Mol Biol Cell 12, 2730–2741.
Wang, J.-C. and Thampatty, P. (2008) Mechanobiology of adult and stem cells. Int Rev Cytol 271, 297-332..
Whitesides, G.M., Ostuni, E., Takayama, S., et al. (2001) Soft lithography in biology and biochemistry. Annu Rev Biomed Eng 3, 335–373.
Acknowledgement
We gratefully acknowledge the funding support of NIH AR049921 and AR049921S1 (JHW).
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Wang, J.HC., Li, B. (2009). Application of Cell Traction Force Microscopy for Cell Biology Research. In: Gavin, R. (eds) Cytoskeleton Methods and Protocols. Methods in Molecular Biology, vol 586. Humana Press. https://doi.org/10.1007/978-1-60761-376-3_17
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DOI: https://doi.org/10.1007/978-1-60761-376-3_17
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