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Variations on the Single-Molecule Assay for Microtubule-Associated Proteins and Kinesins

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Microtubule Dynamics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 777))

Abstract

The direct observation of single kinesins and microtubule-associated proteins (MAPs) has become a core tool for cytoskeleton research. We outline several variations to the core experiment that allow the researcher to explore structural and biophysical mechanisms underlying kinesin motility and MAP function.

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References

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Author information

Authors and Affiliations

  1. Department of Biology, McGill University, Montréal, QC, Canada

    Susanne Bechstedt, Michal Wieczorek, Michael Noujaim & Gary J. Brouhard

Authors
  1. Susanne Bechstedt
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  2. Michal Wieczorek
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  3. Michael Noujaim
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  4. Gary J. Brouhard
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Corresponding author

Correspondence to Gary J. Brouhard .

Editor information

Editors and Affiliations

  1. Warwick Medical School, Ctr for Mechanochemical Cell Biology, University of Warwick, Coventry, CV4 7AL, United Kingdom

    Anne Straube

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© 2011 Springer Science+Business Media, LLC

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Bechstedt, S., Wieczorek, M., Noujaim, M., Brouhard, G.J. (2011). Variations on the Single-Molecule Assay for Microtubule-Associated Proteins and Kinesins. In: Straube, A. (eds) Microtubule Dynamics. Methods in Molecular Biology, vol 777. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-252-6_12

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  • DOI: https://doi.org/10.1007/978-1-61779-252-6_12

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-251-9

  • Online ISBN: 978-1-61779-252-6

  • eBook Packages: Springer Protocols

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