Abstract
Artificial nucleases have developed into powerful tools for introducing precise genome modifications in a wide variety of species. In this chapter the authors provide detailed protocols for rapidly constructing zinc finger nucleases (ZFNs) and TALE nucleases (TALENs) and evaluating their activity for the targeted generation of InDels within the zebrafish genome.
Key words
Fatma O. Kok and Ankit Gupta contributed equally to this work.
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Kok, F.O., Gupta, A., Lawson, N.D., Wolfe, S.A. (2014). Construction and Application of Site-Specific Artificial Nucleases for Targeted Gene Editing. In: Ochs, M. (eds) Gene Function Analysis. Methods in Molecular Biology, vol 1101. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-721-1_13
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DOI: https://doi.org/10.1007/978-1-62703-721-1_13
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