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Super-Resolution Fluorescence Microscopy Using Structured Illumination

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Super-Resolution Microscopy Techniques in the Neurosciences

Part of the book series: Neuromethods ((NM,volume 86))

Abstract

The resolution of far-field fluorescence microscopy is limited by the Abbe diffraction limit. Making use of the moiré effect, structured illumination microscopy circumvents this limit by projecting fine patterns of light into the sample. From several diffraction limited raw images taken for different pattern positions and orientations, a high resolution image can be calculated. This way, linear structured illumination can enhance the resolution by a factor of about two. Employing nonlinearities such as fluorescence saturation, the resolution can be enhanced even further. In this article, a conceptual as well as a mathematical introduction to the technique is provided, as well as several examples of applications.

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Author information

Authors and Affiliations

  1. Institute of Physical Chemistry, Friedrich-Schiller-University, Jena, Germany

    Kai Wicker

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  1. Kai Wicker
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Editor information

Editors and Affiliations

  1. Department of Neuro- and Sensory Physiology,University of Göttingen Medical Center, Göttingen, Germany

    Eugenio F. Fornasiero

  2. Department of Neuro- and Sensory Physiology,University of Göttingen Medical Center, Göttingen, Germany

    Silvio O. Rizzoli

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Wicker, K. (2014). Super-Resolution Fluorescence Microscopy Using Structured Illumination. In: Fornasiero, E., Rizzoli, S. (eds) Super-Resolution Microscopy Techniques in the Neurosciences. Neuromethods, vol 86. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-983-3_7

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  • DOI: https://doi.org/10.1007/978-1-62703-983-3_7

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-982-6

  • Online ISBN: 978-1-62703-983-3

  • eBook Packages: Springer Protocols

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