Summary
The effects of recombinant DNA propagation and gene expression on the physiology of the host cell was investigated using a series of copy number mutant plasmids. The plasmids at copy numbers of 30, 57, 115 and 501 per chromosome equivalent encoded constitutive production of the enzyme β-lactamase. Ribose phosphate isomerase activity was relatively unaffected by plasmid presence, and glucose-6-phosphate dehydrogenase, fructose 1,6-diphosphate aldolase and fructose 1,6-diphosphatase activities were lower in plasmid-containing cells than in the plasmid-free host strain. Increasing copy number resulted in increased depression of enzyme activity levels. The results indicate that plasmid presence mediates subtle changes in the net expression of host enzymes involved in carbon metabolism. Responses of Escherichia coli DH5α in Evans medium to these plasmids differed substantially from responses of E. coli HB101 in rich medium.
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Anthony Mason, C., Bailey, J.E. Effects of plasmid presence on growth and enzyme activity of Escherichia coli DH5α. Appl Microbiol Biotechnol 32, 54–60 (1989). https://doi.org/10.1007/BF00164823
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DOI: https://doi.org/10.1007/BF00164823