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Cell type-dependent difference in the distribution and frequency of aphidicolin-induced fragile sites: T and B lymphocytes and bone marrow cells

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Summary

The distribution and frequency of aphidicolin-induced common fragile sites were studied in Epstein-Barr virus-transformed B lymphocytes from eight normal individuals, and in bone marrow cells from six children in remission from malignant blood diseases. PHA-stimulated helper T lymphocytes from the same individuals were also studied. These cells were cultured in MEM, and treated with 0.2μM aphidicolin for 26 h. The results, together with those of our previous study on cultured skin fibroblasts, indicated that the distribution and frequency of aphidicolin-induced fragile sites are different among different types of cells.

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Authors and Affiliations

  1. Department of Pediatrics, Yamaguchi University School of Medicine, Ube, 755, Yamaguchi-ken, Japan

    Ichiro Murano, Akira Kuwano & Tadashi Kajii

Authors
  1. Ichiro Murano
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  2. Akira Kuwano
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  3. Tadashi Kajii
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Murano, I., Kuwano, A. & Kajii, T. Cell type-dependent difference in the distribution and frequency of aphidicolin-induced fragile sites: T and B lymphocytes and bone marrow cells. Hum Genet 84, 71–74 (1989). https://doi.org/10.1007/BF00210675

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  • DOI: https://doi.org/10.1007/BF00210675

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