Summary
The nucleotide sequence of an IS1 element recently transposed into the lacI gene is reported. This sequence is nearly identical to one previously reported for another IS1 element (Ohtsubo and Ohtsubo, 1978). The implications of this similarity are discussed. The sizes of potential polypeptides encoded in the IS1 DNA have been determined and possible roles for these peptides in the illegitimate recombination events mediated by the element are considered.
Similar content being viewed by others
References
Adhya, S.L., Shapiro, J.A.: The galactose operon of E. coli K12. I. Structural and pleiotropic mutations of the operon. Genet. 62, 231–247 (1969)
Bukhari, A.I., Shapiro, J.A., Adhya, S.L.: DNA insertion elements, plasmids, and episomes. New York: Cold Spring Harbor Laboratory 1977
Calos, M.: DNA sequence for a low-level promotor of the lac repressor gene and an “up” promotor mutation. Nature 274, 762–765 (1978)
Calos, M., Johnsrud, L., Miller, J.H.: DNA sequence at the integration sites of the insertion element IS1. Cell 13, 411–418 (1978)
Cohen, S.N.: Transposable genetic elements and plasmid evolution. Nature 263, 731–738 (1976)
Farabaugh, P. J.: Sequence of the lacI gene. Nature 274, 765–769 (1978a)
Farabaugh, P.J.: The lacI Gene. Ph.D. Thesis, Harvard University (1978b)
Grindley, N.D.F.: IS1 insertion generates duplication of a nine base pair sequence at its target site. Cell 13, 419–426 (1978)
Heffron, F., Bedinger, P., Champoux, J.J., Falkow, S.: Deletions affecting the transposition of an antibiotic resistance gene. Proc. Natl. Acad. Sci. U.S.A. 74, 702–706 (1977)
Jordan, E., Saedler, H., Starlinger, P.: Strong polar mutations in the transferase gene of the galactose operon in E. coli. Mol. Gen. Genet. 100, 296–306 (1967)
Kamen, R.: Characterization of the subunits of Qβ replicase. Nature 228, 527–533 (1970)
Kleckner, N.: Translocatable elements in procaryotes. Cell 11, 11–23 (1977)
Kondo, M., Gallerani, R., Weissman, C.: Subunit structure of Qβ replicase. Nature 228, 525–526 (1970)
MacHattie, L.A., Shapiro, J.A.: Chromosomal integration of phage lambda by means of a DNA insertion element. Proc. Natl. Acad. Sci. U.S.A. 75, 1490–1494 (1978)
Maxam, A., Gilbert, W.: A new method for sequencing DNA. Proc. Natl. Acad. Sci. U.S.A. 74, 560–564 (1977)
Nash, H.: Integration and excision of bacteriophage lambda. Curr. Top. Microbiol. Immunol. 78, 171–199 (1977)
Nevers, P., Saedler, H.: Transposable genetic elements as agents of gene instability and chromosomal rearrangements. Nature 268, 109–115 (1977)
Nisen, P.D., Kopecko, D.J., Chou, J., Cohen, S.N.: Site specific deletions occurring adjacent to the termini of a transposable ampicillin resistance element (Tn3). J. Mol. Biol. 117, 975–998 (1977)
Ohtsubo, H., Ohtsubo, E.: Nucleotide sequence of an insertion element, IS1. Proc. Natl. Acad. Sci. U.S.A. 75, 615–619 (1978)
Pirotta, V.: Sequence of the OR operator of phage lambda. Nature 254, 114–117 (1975)
Pribnow, D.: Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter. Proc. Natl. Acad. Sci. U.S.A. 72, 784–788 (1975)
Reif, H.J., Saedler, H.: IS1 is involved in deletion formation in the gal region of E. coli K12. Mol. Gen. Genet. 137, 17–28 (1975)
Reif, H.J., Saedler, H.: Chromosomal rearrangements in the gal region of E. coli K12 after integration of IS1. In: DNA insertion elements, plasmids, and episomes (Bukhari, A., Shapiro, J., and Adhya, S., eds), pp. 81–91. New York: Cold Spring Harbor Laboratory 1977
Saedler, H., Starlinger, P.: 0° mutations in the galactose operon in E. coli. I. Genetic characterization. Mol. Gen. Genet. 100, 178–189 (1967a)
Saedler, H., Starlinger, P.: 0° mutations in the galactose operon in E. coli. II. Physiological characterization. Mol. Gen. Genet. 100, 190–202 (1967b)
Sanger, F., Coulson, A.R.: A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. J. Mol. Biol. 94, 441–448 (1975)
Schaller, H., Gray, C., Hermann, K.: Nucleotide sequence of an RNA polymerase binding site from the DNA of bacteriophage fd. Proc. Natl. Acad. Sci. U.S.A. 72, 737–741 (1975)
Sekiya, R., Takeya, T., Coutreras, R., Kupper, H., Khorana, H., Landy, A.: Nucleotide sequences at the two ends of the E. coli tyrosine tRNA genes and studies on the promoter. In: RNA polymerase (R. Losick, M. Chamberlin, eds.), pp. 455–472. New York: Cold Spring Harbor Laboratory 1976
Shapiro, J., Adhya, S.: The galactose operon of E. coli K12. II. A deletion analysis of operon structure and polarity. Genet. 62, 249–264 (1969)
Shine, J., Dalgarno, L.: The 3′ terminal sequence of E. coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc. Natl. Acad. Sci. U.S.A. 71, 1342–1346 (1974)
Starlinger, P., Saedler, H.: IS elements in microorganisms. Curr Top. Microbiol. Immunol. 75, 111–152 (1976)
Steitz, J.A., Jakes, K.: How ribosomes select initiator regions in mRNA: base pair formation between the 3′ terminus of 16S rRNA and the mRNA during initiation of protein synthesis in E. coli. Proc. Natl. Acad. Sci. U.S.A. 72, 4734–4738 (1975)
Walz, A., Pirotta, V.: Sequence of the PR promoter of phage lambda. Nature 254, 118–121 (1975)
Author information
Authors and Affiliations
Additional information
Communicated by E. Bautz
Rights and permissions
About this article
Cite this article
Johnsrud, L. DNA sequence of the transposable element IS1. Molec. Gen. Genet. 169, 213–218 (1979). https://doi.org/10.1007/BF00271673
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00271673