Summary
The EM study of the mouse embryonic cornea from the 12th to the 19th day of gestation as well as on postnatal days 2 and 18 and on adult animals allow the following conclusions to be drawn:
-
1.
Immediately after the separation of the lens vesicle, the mesenchyme cells migrate into the cornea analage.
-
2.
There is no collagenous primary stroma in the mouse embryo.
-
3.
During days 12–14 the stroma cells (fibroblasts) differentiate and develop the organelles required for ICS (intercellular substance) secretion.
-
4.
In the posterior region of the stroma, the collagen fibrils are deposited in bundles approximately perpendicular to each other.
-
5.
The adult mouse stroma is divided into 2 zones. In zone I the subepithelial fibrils are randomly distributed and are not bundled (rudimentary Bowman's membrane). In zone II the fiber bundles lie in the plane of the cornea and form a highly ordered three-dimensional network.
Basic differences between the mouse and other species are discussed.
Similar content being viewed by others
References
Bard JB, Hay LD, Meller SM (1975) Formation of the endothelium of the avian cornea: a study of cell movement in vivo. Dev Biol 42:334–361
Coulombre AJ (1965) Problems in corneal morphogenesis. Adv Morphogenesis 4:81–109
Coulombre AJ, Coulombre J (1961) The development of the structural and optical properties of the cornea. In: Smelser GK (ed) The structure of the eye. Academic Press, New York, pp 405–420
Coulombre J, Coulombre A (1975) Corneal development V. Treatment of five day old embryos of domestic fowl with 6-Diazo-5-Oxo L-Norleucine (DON). Dev Biol 45:291–303
Dueblin I (1970) Vergleichend embryologische Untersuchungen über die Frühentwicklung der Hornhaut und der Pupillarmembran bei Reptilien, Vögeln und Säugern. Acta Anat 76:381–408
Duke-Elder S (1939) Textbook of ophthalmology. Mosby. St. Louis
Ewer MS (1970) Zur Frühentwicklung des stroma corneae und der Pupillarmembran beim Menschen. Acta Anat 75:37–46
Fine BS, Yanoff M (1972) Ocular histology. Harper and Row, New York
Hackelberg HH (1971) Elektronenmikroskopische Untersuchungen über die Entstehung und Reifung des Corneastromas bei der Ratte. Inauguraldissertation FU Berlin
Hagedoorn A (1930) Beitrag zur Entwicklungsgeschichte des Auges. Arch Augenhlkd 102:33–110
Hay ED, Revel J (1969) Fine structure of the developing avian cornea. Karger, Basel, pp 1–144
Jacus MA (1961) The fine structure of the human cornea. In: Smelser GK (ed) The structure of the eye. Academic Press, New York
Jacus MA (1964) Ocular fine structure. Churchill London
Karnowsky MJ (1965) A formaldehyde-glutaraldehyde fixative of high osmolatiy for use in EM. J Cell Biol 27:137A
Laguesse E (1923) Les lamelles primitives de la cornée du poulet sont comme le corps vitre d'origine mesostromale ectodermique. C R Soc Biol (Paris) 89:543–546
Medvedeva A (1955) A comparative-embryological study of the mammalian eye. Thesis Moscow
Merker HJ, Struwe K (1971) Elektronenmikroskopische Untersuchungen zum Problem der Sekretion der bindegewebigen Interzellularsubstanz. Z Zellforsch 115:212–225
O'Rahilly R, Meyer DB (1959) The early development of the eye in the chick. Acta Anat 36:20–58
Ozanics V, Rayborn M, Sagun D (1976) Some aspects of corneal and scleral differentation in the primate. Exp Eye Res 22:305–326
Ozanics V, Rayburn M, Sagun D (1977) Observations on the morphology of the developing primate corneal epithelium, its innervation and anterior stroma. J Morphol 153:263–298
Payrau P, Pouliquen Y, Faure JP, Offret G (1967) Le Transparence de la cornée. Masson et Cie Paris
Pei YF, Rhodin JA (1971) Electron microscopic study of the development of the mouse corneal epithelium. Invest Ophthal 10:811–825
Polack FM (1961) Morphology of the cornea. Am J Ophthalmol 51:1051–1059
Rehbinder C (1978) Fine structure of the mouse cornea. Z Versuchstierkunde 20:28–34
Ross R, Benditt E (1965) Wound healing and collagen formation. J Cell Biol 27:83–106
Schwarz W (1953) Elektronenmikroskopische Untersuchungen über die Differenzierung der Cornea-und Sklerafibrillen des Menschen. Z Zellforsch 38:78–86
Sheldon H (1956) An EM study of the epithelium in the normal mature and immature mouse cornea. J Biophys Biochem Cyt 2:253–262
Trelstad R (1971) Vacuoles in the embryonic chick corneal epithelium, an epithelium which produces collagen. J Cell Biol 689–694
Trelstad R, Coulombre AJ (1971) Morphogenesis of the collagenous stroma in the chick cornea. J Cell Biol 50:840–858
Virchow R (1910) Handbuch der gesamten Augenhlkd, vol 1. Engelmann, Leipzig
Weinstock M, Leblond C (1974) Synthesis, migration and release of precursor collagen by odontoblasts as visualized by radioautography after 3H-proline administration. J Cell Biol 60:92–127
Whitear M (1960) An EM study of the cornea in mice, with special reference to the innervation. J Anat 94:387–408
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Haustein, J. On the ultrastructure of the developing and adult mouse corneal stroma. Anat Embryol 168, 291–305 (1983). https://doi.org/10.1007/BF00315823
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00315823