Abstract
The B mating type of the basidiomycete fungus, Schizophyllum commune is determined by two, tightly linked, multi-specificity (also called multi-allelic) loci: Bα and Bβ. A plasmid library was used in DNA-mediated transformation to obtain transformants that displayed B-directed development. Plasmids that conferred Bα1 and Bβ1 mating-type specificities were rescued from the transformants. Fragments of DNA from each plasmid hybridized to genomic DNA from the strain used to make the plasmid library; however, they did not hybridize, or hybridized only weakly, to genomic DNA from strains with mating-type specificities different from Bα1 or Bβ1. The cloned fragments are presumed to correspond to active regions of each B mating-type locus.
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Communicated by G. Simchen
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Specht, C.A. Isolation of the Bα and Bβ mating-type loci of Schizophyllum commune . Curr Genet 28, 374–379 (1995). https://doi.org/10.1007/BF00326436
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DOI: https://doi.org/10.1007/BF00326436