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Bialaphos resistance as a dominant selectable marker in Neurospora crassa

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Summary

Conidia of Neurospora crassa are sensitive to the herbicide bialaphos at concentrations of 160 mg/1 in Westergaard's or Fries' minimal media. Plasmid pJA4 was constructed by inserting a truncated bar gene from Streptomyces hygroscopicus fused to the his-3 promoter from N. crassa into pUC19. The bar gene in plasmid pJA4 confers resistance to bialaphos when transformants are selected on a medium containing bialaphos. The bar gene can be used as an additional dominant selectable marker for transformation of fungi.

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Author notes

  1. Javier Avalos

    Present address: Department of Genetics, Faculty of Biology, University of Seville, E-41012, Sevilla 12, Spain

Authors and Affiliations

  1. Department of Genetics, University of Georgia, 30602, Athens, GA, USA

    Javier Avalos, Robert F. Geever & Mary E. Case

Authors
  1. Javier Avalos
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  2. Robert F. Geever
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  3. Mary E. Case
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Avalos, J., Geever, R.F. & Case, M.E. Bialaphos resistance as a dominant selectable marker in Neurospora crassa . Curr Genet 16, 369–372 (1989). https://doi.org/10.1007/BF00340716

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  • DOI: https://doi.org/10.1007/BF00340716

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