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Minimal methylation of yeast messenger RNA

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Abstract

Most, if not all, yeast mRNAs are capped at their 5′-terminus by m7G. Apart from m7G no other methylated nucleotides could be detected in poly (A)+ mRNA isolated from yeast polysomes.

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Abbreviations

poly (A)+ mRNA:

messenger RNA containing poly (A)

poly (A)- RNA:

RNA lacking poly (A)

m7G:

N7-methyl guanosine

Nm :

any 2′-0 methylated nucleoside

mN:

any basemethylated nucleoside

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Authors and Affiliations

  1. Biochemisch Laboratorium, Free University, Amsterdam, The Netherlands

    W. H. Mager, J. Klootwijk & I. Klein

Authors
  1. W. H. Mager
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  2. J. Klootwijk
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  3. I. Klein
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Mager, W.H., Klootwijk, J. & Klein, I. Minimal methylation of yeast messenger RNA. Molecular Biology Reports 3, 9–17 (1976). https://doi.org/10.1007/BF00357204

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  • DOI: https://doi.org/10.1007/BF00357204

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