Summary
A new mutation has been described which confers resistance to catabolite repression in Saccharomyces cerevisiae. The mutant allele, termed grr-1 for glucose repression-resistant, is characterized by insensitivity to glucose repression for the cytoplasmic enzymes invertase, maltase, and galactokinase, as well as the mitochondrial enzyme cytochrome c oxidase. Hexokinase levels in grr-1 mutants are approximately 3-fold higher than the corresponding activity of the parental strain. Although the grr-1 allele is expressed phenotypically similarly to the hex-1 (hxk-2) and hex-2 mutations described by Entian et al. (1977) and Zimmermann and Scheel (1977) respectively, we have shown genetically and physiologically that grr-1 represents a new class of mutation.
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References
Adams BG (1972) Induction of galactokinase in Saccharomyces cerevisiae: kinetics of induction and glucose effects. J Bacteriol 111:308–315
Bailey RB, Benitez T, Woodward A (1982) Saccharomyces cerevisiae mutants resistant to catabolite repression: Use in cheese whey hydrolysate fermentation. Appl Environ Microbiol 44:631–639
Böker-Schmitt E, Francisci S, Schweyen RJ (1982) Mutations releasing mitochondrial biogenesis from glucose repression in Saccharomyces cerevisiae. J Bacteriol 151:303–310
Ciriacy M (1978) A yeast mutant with glucose-resistant formation of mitochondrial enzymes. Mol Gen Genet 129:329–335
de Crombrugghe B, Pastan I (1978) Cyclic AMP, the cyclic AMP receptor protein and their dual control of the galactose operon. In: Miller JH, Reznikoff WS (eds) The operon. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, p 303–324
Entian KD, Zimmermann FK, Scheel I (1977) A partial defect in carbon catabolite repression in mutants of Saccharomyces cerevisiae with reduced hexose phosphorylation. Mol Gen Genet 156:99–105
Entian KD (1980a) Genetic and biochemical evidence for hexokinase PII as a key enzyme involved in carbon catabolite repression in yeast. Mol Gen Genet 178:633–637
Entian KD (1980b) A defect in carbon catabolite repression associated with uncontrollable and excessive maltose uptake. Mol Gen Genet 179:169–175
Gascón S, Neumann NP, Lampen JO (1968) Comparative study of the properties of the purified internal and external invertases from yeast. J Biol Chem 243:1573–1577
Lemont JF (1977) Genetic analysis and properties of mutants resistant to ultraviolet induced forward mutation. Mutat Res 43:179–204
Lobo Z, Maitra PK (1977) Genetics of yeast hexokinase. Genetics 86:727–744
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Magasanik B (1961) Catabolite repression. Cold Spring Harbor Symp Quant Biol 26:249–256
Michels CA, Romanowski A (1980) Pleiotropic glucose repression-resistant mutation in Saccharomyces carlesbergensis J Bacteriol 143:674–679
Montenecourt BS, Kuo SC, Lampen JO (1973) Saccharomyces mutants with invertase formation resistant to repression by hexoses. J Bacteriol 114:233–238
Polakis ES Bartley W (1965) Changes in the enzyme activities of Saccharomyces cerevisiae during aerobic growth on different carbon sources. Biochem J 97:284–297
Polakis ES, Bartley W, Meek GA (1965) Changes in the activities of respiratory enzymnes during the aerobic growth of yeast on different carbon sources. Biochem J 97:298–302
Rothstein RJ, Sherman F (1980) Genes affecting the expression of cytochrome c in yeast: Genetic mapping and genetic interactions. Genetics 94:871–889
Schamhart DHJ, ten Berge AMH, van de Poll KW (1975) Isolation of a catabolite repression mutant of yeast as a revertant of a strain that is maltose negative in the respiratory-deficient state. J Bacteriol 121:747–752
Sherman F, Fink GR, Lawrence CW (1979) Methods in yeat genetics, laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Stark HC, Fugit D, Mowshowitz DB (1980) Pleiotropic properties of a yeast mutant insensitive to catabolite repression. Genetics 94:921–928
Thompson ED, Bailey RB, Parks LW (1974) Subcellular location of s-adenosylmethionine: Δ24-sterol methyltransferase in Saccharomyces cerevisiae. Biochim Biophys Acta 334:116–126
Wickner R (1974) Mutants of Saccharomyces cerevisiae that incorporate deoxythymidine-5′-monophosphate into deoxyribonucleic acid. J Bacteriol 117:242–260
Zimmermann FK, Eaton NR (1974) Genetics of induction and catabolite repression of maltase synthesis in Saccharomyces cerevisiae. Mol Gen Genet 134:261–272
Zimmermann FK, Kaufmann I, Rasenberger H, Haussmann P (1977) Genetics of carbon catabolite repression in Saccharomyces cerevisiae: Genes involved in the derepression process. Mol Gen Genet 151:95–103
Zimmermann FK, Scheel I (1977) Mutants of Saccharomyces cerevisiae resistant to carbon catabolite repression. Mol Gen Genet 154:75–82
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Communicated by G.A. O'Donovan
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Bailey, R.B., Woodword, A. Isolation and characterization of a pleiotropic glucose repression resistant mutant of Saccharomyces cerevisiae . Molec. Gen. Genet. 193, 507–512 (1984). https://doi.org/10.1007/BF00382091
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DOI: https://doi.org/10.1007/BF00382091