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A rapid gas chromatographic method for the determination of poly-β-hydroxybutyric acid in microbial biomass

  • Biotechnology and Bioengineering
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European journal of applied microbiology and biotechnology Aims and scope Submit manuscript

Summary

The gas chromatographic method for the determination of poly-β-hydroxybutyric acid (PHB) consists of a mild acid or alkaline methanolysis of poly-β-hydroxybutyric acid directly without previous extraction of PHB from the cells; this is followed by gas chromatography of the 3-hydroxybutyric acid methylester. The method is characterized by high accuracy and excellent reproducibility, permitting determinations as low as 10−5 g/l. Only 4 h is required from sampling from the fermenter till completion of the PHB determination.

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References

  • Cornibert, V., Marchessault, R.H. (1972): J. Mol. Biol. 71, 735–756

    Google Scholar 

  • Dawes, E.A., Senior, P.J. (1973): Adv. Microb. Physiol. 10, 135–266

    Google Scholar 

  • Fukui, T., Yoshimoto, A., Matsumoto, M., Hosokawa, S., Saito, T., Nishikawa, H., Tomito, K. (1976): Arch. Microbiol. 110, 149–156

    Google Scholar 

  • Heinzle, E., Lafferty, R.M. (1977): Chem. Rundschau 30 (41), 14–16

    Google Scholar 

  • Jüttner, R.R., Lafferty, R.M., Knackmuss, H.J. (1975): Europ. J. Appl. Microbiol. 1, 233–237

    Google Scholar 

  • Lemoigne, M. (1926): Bull. Soc. Chim. Biol. 8, 770

    Google Scholar 

  • Lundgren, D.G., Alper, R., Schnaitman, C., Marchessault, R.H. (1965): J. Bact. 89 (1), 245–251

    Google Scholar 

  • Moser, A., Lafferty, R.M. (1976): 5th Intern. Ferment. Sympos., Berlin, Abstr. p. 103

  • Schlegel, H.G., Gottschalk, G., Kaltwasser, H. (1961): Arch. Microbiol. 38, 209–222

    Google Scholar 

  • Schwab, H. (1977): Personal Communication

  • Siapkas, S. (1976): Diplomarbeit T.U. Graz

  • Slepecky, R.A., Law, J.H. (1969): Anal. Chem. 32, 1697–1699

    Google Scholar 

  • Srienc, F. (1977): Personal Communication

  • Ward, A.C., Dawes, E.A. (1973): Anal. Biochem. 52, 607–613

    Google Scholar 

  • Wilde, E. (1962): Arch. Microbiol. 43, 109–137

    Google Scholar 

  • Williamson, D.H., Wilkinson, J.F. (1958): J. Gen. Microbiol. 19, 198–209

    Google Scholar 

Download references

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Authors and Affiliations

  1. Institut für Biochemische Technologie und Lebensmittelchemie der Technischen Universität Graz, Schlögelgasse 9, A-8010, Graz, Austria

    G. Braunegg, B. Sonnleitner & R. M. Lafferty

Authors
  1. G. Braunegg
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  2. B. Sonnleitner
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  3. R. M. Lafferty
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Braunegg, G., Sonnleitner, B. & Lafferty, R.M. A rapid gas chromatographic method for the determination of poly-β-hydroxybutyric acid in microbial biomass. European J. Appl. Microbiol. Biotechnol. 6, 29–37 (1978). https://doi.org/10.1007/BF00500854

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  • DOI: https://doi.org/10.1007/BF00500854

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