Summary
The gas chromatographic method for the determination of poly-β-hydroxybutyric acid (PHB) consists of a mild acid or alkaline methanolysis of poly-β-hydroxybutyric acid directly without previous extraction of PHB from the cells; this is followed by gas chromatography of the 3-hydroxybutyric acid methylester. The method is characterized by high accuracy and excellent reproducibility, permitting determinations as low as 10−5 g/l. Only 4 h is required from sampling from the fermenter till completion of the PHB determination.
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Braunegg, G., Sonnleitner, B. & Lafferty, R.M. A rapid gas chromatographic method for the determination of poly-β-hydroxybutyric acid in microbial biomass. European J. Appl. Microbiol. Biotechnol. 6, 29–37 (1978). https://doi.org/10.1007/BF00500854
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DOI: https://doi.org/10.1007/BF00500854