Summary
Treatment with lysine prior to fixation of tobacco BY-2 cells with formaldehyde improved the preservation of actin filaments in the cells and enabled us to observe both networks of actin filaments and microtubules in the same cells. By using this method, we observed that (1) actin filaments were present in the preprophase band; (2) the actin filaments in the preprophase band and phragmoplast were runnig in the same direction as the microtubules in their respective structures; (3) a cortical network of actin filaments was present throughout all stages of cell cycle.
The present method did not preserve the cortical actin filaments in interphase cells. The procedure for staining microtubules destroyed them.
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Abbreviations
- EGTA:
-
Ethyleneglycol-bis(β-aminoethyl ether)N,N,N′,N′-tetraacetic acid
- PIPES:
-
Piperazine-N,N′-bis(2-ethanesulfonic acid)
- PMSF:
-
Phenylmethylsulfonyl fluoride
- TLCK:
-
Na-p-tosyl-L-lysine chloromethyl ketone
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Kakimoto, T., Shibaoka, H. Actin filaments and microtubules in the preprophase band and phragmoplast of tobacco cells. Protoplasma 140, 151–156 (1987). https://doi.org/10.1007/BF01273724
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DOI: https://doi.org/10.1007/BF01273724