Summary
The volumes of whole cells and nuclei of cultured human cells were studied at different times after synchronization of growth using the Coulter counter and scanning microphotometry. It was found that the increase in cell volume is compatible with both linear or exponential growth during the cell cycle. The growth of the nuclear volume is not correlated with the beginning of the DNA synthesis. The nuclear volume starts to increase already 6 h prior DNA synthesis. The data also indicate that the nuclear volume growth could proceed in two stages. The relation of this result to radiation sensitivity is discussed.
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References
Anderson, E. C., Petersen, D. F.: Cell growth and division. II. Experimental studies of cell volume distribution in mammalian suspension cultures. Biophys. J.7, 353–364 (1967)
Bell, G. I., Anderson, E. C.: Cell growth and division. I. Mathematical model with application to cell volume distributions in mammalian suspension cultures. Biophys. J.7, 329–351 (1967)
Bonner, J. T., Eden, M.: The form of the frequency distribution curve of cell and nuclear sizes. Exp. Cell Res.11, 265–269 (1956)
Cavalier-Smith, T.: Nuclear volume control by nucleoskeletal DNA, selection for cell volume and cell growth rate, and the solution of the DNA C-value paradox. J. Cell Sci.34, 247–278 (1978)
Dettor, C. M., Dewey, W. C., Winans, L. F., Noel, J. S.: Enhancement of X-ray damage in synchronous Chinese hamster cells by hypertonic treatment. Radiat. Res.52, 352–372 (1972)
Dewey, W. C., Noel, J. S., Dettor, C. M.: Changes in radiosensitivity and dispersion of chromatin during the cell cycle of synchronous Chinese hamster cells. Radiat. Res.30, 373–394 (1972)
Elkind, M. M.: Damage and repair processes relative to neutron (and charged particle) irradiation. In: Current topics in radiation research. Ebert, M., Howard, A. (eds.), pp. 1–44. Amsterdam: North Holland Publishing Company 1974
Fidorra, J.: Die Strahlenempfindlichkeit von Saugetierzellen in den verschiedenen Zyklusphasen und ihre Beziehung zu Erholungsprozessen - Experimentelle Untersuchungen und Erstellung eines mathematischen Modells. Thesis, Hamburg (1977)
Fidorra, J., Linden, W. A.: Radiosensitivity and recovery of mouse L cells during the cell cycle. Radiat. Environ. Biophys.14, 285–294 (1977)
Galavazi, G., Bootsma, D.: Synchronization of mammalian cells in vitro by inhibition of the DNA synthesis. II. Population dynamics. Exp. Cell Res.41, 438–451 (1966)
Gerner, E. W., Humphrey, R. M.: The cell cycle phase synthesis of non-histone proteins in mammalian cells. Biochim. Biophys. Acta331, 117–127 (1973)
Gregg, E. C., Steidly, K. D.: Electrical counting and sizing of mammalian cells in suspension. Biophys. J.5, 393–405 (1965)
Gurley, L. R., Walters, R. A., Tobey, R. A.: Cell cycle-specific changes in histone phosphorylation associated with cell proliferation and chromosome condensation. J. Cell Biol.60, 356–364 (1974)
Kendall, F., Swenson, R., Boron, T., Rawinski, F., Nicolini, C.: Nuclear morphometry during the cell cycle. Science196, 1106–1109 (1977)
Peacock, A. C., Williams, G. Z., Mengoli, H. F.: Rapid electronic measurements of cell volume and distribution. J. Natl. Cancer Inst.25, 63–74 (1960)
Rosenberg, H. M., Gregg, E. C.: Kinetics of cell volume changes of murine lymphoma cells subjected to different agents in vitro. Biophys. J.9, 592–606 (1969)
Steen, H. B., Lindmo, T.: Cellular and nuclear volume during the cell cycle of NHIK 3025 cells. Cell Tissue Kinet.11, 69–81 (1978)
Underbrink, A. G., Sparrow, A. H., Bond, V.: Chromosome and cellular radiosensitivity II. Use of interrelationships among chromosome volume, nucleotid content, and D0 of 120 diverse organisms in predicting radiosensitivity. Radiat. Botany8, 205–237 (1968)
Watanabe, I., Okada, S.: Effects of temperature on growth rate of cultured mammalian cells (L5178Y). J. Cell Biol.32, 309–317 (1967)
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This research was carried out under contract no. 215-76-10-BIO-D, Radiation Protection programme of the Commission of the European Community (Publication no. BIO 1747)
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Fidorra, J., Mielke, T., Booz, J. et al. Cellular and nuclear volume of human cells during the cell cycle. Radiat Environ Biophys 19, 205–214 (1981). https://doi.org/10.1007/BF01324188
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DOI: https://doi.org/10.1007/BF01324188