Summary
The mitochondria of living mouse fibroblasts infected withToxoplasma gondii were monitored with the cationic permeant fluorescent dye rhodamine 123. Fluorescence microscopy revealed that host cell mitochondria accumulated at the cytoplasmic surface of parasitophorous vacuoles and increased the dye uptake in the periparasitophorous vacuole asT. gondii multiplied.
Literatur
Jones, T. C., and Hirsh, J. G., J. exp. Med.136 (1972) 1173.
Johnson, L. V., Walsh, M. L., and Chen, L. B., Proc. natl Acad. Sci. USA77 (1980) 990.
Johnson, L. V., Walsh, M. L., Bockus, B. J., and Chen, L. B., J. Cell Biol.88 (1981) 526.
Tanabe, K., Kimata, I., Tabuse, Y., Furusawa, M., and Takada, S., Exp. Parasit.46 (1978) 72.
Tanabe, K., Protozoology30 (1983) 707.
Sheffield, H. G., and Melton, M. L., J. Parasit.54 (1968) 209.
Tanabe, K., Kimata, I., and Takada, S., J. gen. Microbiol.118 (1980) 45.
Schwartzman, J. D., and Pfefferkorn, E. R., Exp. Parasit.53 (1982) 77.
Tanabe, K., and Murakami, K., J. Cell Sci. (in press).
Author information
Authors and Affiliations
Additional information
The author wishes gratefully to acknowledge the work of Miss Mayumi Kato and Mr. Isao Kimata in preparing the photographs. The publication cost was supported by a grant of the Ohyama Health Foundation.
Rights and permissions
About this article
Cite this article
Tanabe, K. Visualization of the mitochondria ofToxoplasma gondii-infected mouse fibroblasts by the cationic permeant fluorescent dye rhodamine 123. Experientia 41, 101–102 (1985). https://doi.org/10.1007/BF02005897
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02005897