Abstract
A mutation in thelet-653 gene ofCaenorhabditis elegans results in larval death. The lethal arrest is concurrent with the appearance of a vacuole anterior to the lower pharyngeal bulb. The position of the vacuole is consistent with a dysfunction of the secretory/excretory apparatus. Germline transformation rescue experiments were able to position thelet-653 gene to two overlapping cosmid subclones. Sequence data generated from both cDNA and genomic DNA subclones indicated thatlet-653 encodes a mucin-like protein. Our characterization suggests that a mucin-like protein is essential for effective functioning of the secretory/excretory apparatus withinC. elegans.
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Jones, S.J.M., Baillie, D.L. Characterization of thelet-653 gene inCaenorhabditis elegans . Molec. Gen. Genet. 248, 719–726 (1995). https://doi.org/10.1007/BF02191712
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DOI: https://doi.org/10.1007/BF02191712