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Effect of polyclonal immunoglobulins on neutrophil phagocytic capacity and reactive oxygen production in patients with gram-negative septicemia

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The effect of immunglobulin (Ig) preparations on neutrophil phagocytic ability and oxidative burst in response toEscherichia coli stimulation was analyzed in 14 patients with gram-negative septicemia by anex vivo whole blood assay using flow cytometry. In patients, neutrophils exhibited a decreased capacity to phagocytizeE. coli and generate reactive oxygen products compared to healthy controls (median −68%, P<0.01). The addition of both 7S-Ig and 19S-Ig enriched preparationsin vitro resulted in a dose-dependent increase in neutrophil reactive oxygen production at concentrations of 10 g/l (median +153% and +211%, P<0.01, respectively) and 20 g/l (median +205% and +282%, P<0.01, respectively). A decreased neutrophil phagocytic ability was seen in patients with septicemia (median −58%) compared to healthy controls (P<0.01). Again, the addition of 7S and 19S-Igs enhanced the phagocytic ability in a dose-dependent manner (10 g/l: median +56 and +126%; 20 g/l: median +126% and +165%, P<0.01 for all). It can be concluded that both polyclonal Igs can increase depressed neutrophil reactive oxygen production and neutrophil phagocytosis in patients with gram-negative septicemia.

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Authors and Affiliations

  1. Abt. für Infektionen, Universitätsklinik für Innere Medizin 1, Währinger Gürtel 18-20, A-1090, Wien, Austria

    C. Wenisch,  B. Parschalk,  S. Patruta &  W. Graninger

  2. II. Medizinische Abt., Krankenhaus St. Pölten, St. Pölten, Austria

    R. Brustbauer

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  1. C. Wenisch
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  2. B. Parschalk
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  3. S. Patruta
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  4. R. Brustbauer
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  5. W. Graninger
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Wenisch, C., Parschalk, B., Patruta, S. et al. Effect of polyclonal immunoglobulins on neutrophil phagocytic capacity and reactive oxygen production in patients with gram-negative septicemia. Infection 27, 183–186 (1999). https://doi.org/10.1007/BF02561525

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  • DOI: https://doi.org/10.1007/BF02561525

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