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Relationship between in vitro growth promotion and biophysical and biochemical properties of the serum supplement

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Summary

The normal range of many chemical, physical, and biological (hormonal) properties of fetal bovine, calf, and newborn calf sera collected commercially are presented. We attempt to relate these properties to growth promotion in vitro. Whereas no definitive conclusions could be drawn from the data, high growth hormone levels, low endotoxin levels, and low levels of bilirubin seemed to have some association with good growth promotion if the levels of the other parameters were close to the mean. Large variations from the mean of any of the parameters seemed to affect the growth properties of the serum.

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References

  1. Honn, K. V.; Singley, J. A.; Chavin, W. Fetal bovine serum: a multivariate standard. Proc. Soc. Exp. Biol. Med. 149: 344–347; 1975.

    PubMed  CAS  Google Scholar 

  2. Macleod, A. J.; Drummond, O. Serum quality: an analysis of its components. Dev. Biol. Stand. 46: 17–20; 1980.

    PubMed  CAS  Google Scholar 

  3. Olmstead, C. A. A physio-chemical study of fetal calf sera used as a tissue culture nutrient correlated with biological tests for toxicity. Exp. Cell Res. 48: 283–299; 1967.

    Article  Google Scholar 

  4. Bradley, S. G. Cellular and molecular mechanisms of action of bacterial endotoxins. Ann. Rev. Microbiol. 33: 67–94; 1979.

    Article  CAS  Google Scholar 

  5. Berry, L. J. Bacterial toxins. CRC Crit. Rev. Toxicol. 5: 239–318; 1977.

    Article  PubMed  CAS  Google Scholar 

  6. Shands, J. Affinity of endotoxins for membranes. J. Infect. Dis. 128: S197-S201; 1973.

    CAS  Google Scholar 

  7. Rosenstreich, D. L.; Vogel, S. N.; Jacques, A. R.; Wahl, L. M.; Oppenheim, J. J. Macrophage sensitivity to endotoxin: genetic control by a single codominant gene. J. Immunol. 121: 1644–1670; 1978.

    Google Scholar 

  8. Donahoe, R. M.; Peters, R. L. Inconsistency of cell-mediated immunological assays when viral reagents contain endotoxin. Proc. Soc. Exp. Biol. Med. 160: 1–5; 1979.

    PubMed  CAS  Google Scholar 

  9. Fox, R. A.; Rajaraman, K. Endotoxin and macrophage-migration inhibition. Cell Immunol. 53: 333–340; 1980.

    Article  PubMed  CAS  Google Scholar 

  10. McCallum, R. E.; Berry, L. J. Effects of endotoxin on gluconeogenesis, glycogen synthesis, and liver glycogen synthase in mice. Infect. Immunol. 7: 642–654; 1973.

    CAS  Google Scholar 

  11. Ryan, N. T.; Blackburn, G. L.; Clowes, G. H. A. Differential tissue sensitivity to elevated endogenous insulin levels during experimental peritonitis in rats. Metabolism 23: 1081–1089; 1974.

    Article  PubMed  CAS  Google Scholar 

  12. Fundamentals of clinical chemistry. Teitz, N. W. ed.; Philadelphia: W. B. Saunders; 1976.

    Google Scholar 

  13. Yamane, I.; Murakami, O.; Kato, M. Role of bovine albumin in a serum-free suspension cell culture medium. Proc. Soc. Exp. Biol. Med. 149: 439–442; 1975.

    PubMed  CAS  Google Scholar 

  14. Nilausen, K. Role of fatty acids in growth-promotion effect of serum albumin on hamster cells in vitro. J. Cell Physiol. 96: 1–14; 1978.

    Article  PubMed  CAS  Google Scholar 

  15. Rottem, S.; Cirillo, V. P.; DeKruyff, B.; Shinitzky, M.; Razin, S. Correlation of enzymic and transport activities with physical state of lipids in membranes ofMycoplasma mycoides var. capri adapted to grow with low cholesterol concentrations. Biochim. Biophys. Acta 323: 509–519; 1973.

    Article  PubMed  CAS  Google Scholar 

  16. Mather, J.; Sato, G. Hormones and growth factors in cell cultures: problems and perspectives. Federoff, S.; Hertz, L. eds. Cell, tissue and organ cultures in neurobiology. New York: Academic Press; 1978: 619–630.

    Google Scholar 

  17. Bottenstein, J.; Hayashi, I.; Hutchings, S.; Masui, H.; Mather, J.; McClure, D. B.; Ohasa, S.; Rizzino, A.; Sato, G.; Serrero, G.; Wolfe, R.; Wu, R. The growth of cells in serum-free hormone-supplemented media. Methods Enzymol. 58: 94–109; 1970.

    Article  Google Scholar 

  18. Mather, J. P.; Sato, G. H. The use of hormone-supplemented serum-free media in primary cultures. Exp. Cell Res. 124: 215–221; 1979.

    Article  PubMed  CAS  Google Scholar 

  19. Barnes, D.; Sato, G. H. Methods for growth of cultured cells in serum-free medium. Anal. Biochem. 102: 255–270; 1980.

    Article  PubMed  CAS  Google Scholar 

  20. Kano-Sueoka, T.; Errick, J. E.; Cohen, D. M. Effects of hormones and a novel mammary growth factor on a rat mammary carcinoma in culture. Ross, R.; Sato, G. eds. Hormones and cell culture. Cold Spring Harbor conferences on cell proliferation. Vol. 6; New York: Cold Spring Harbor Laboratory; 1979: 499–512.

    Google Scholar 

  21. Samuels, H. H.; Stanley, F.; Shapiro, L. E.; Casanova, J.; Horowitz, Z. D.; Klien, D.; Shao, A.; Yaffe, B. M. Multihormonal control of the growth hormone genome in cultured GH1 cells by thyroid and glucocorticoid hormones. Rose, R.; Sato, G. eds. Hormones and cell culture. Cold Spring Harbor conferences on cell proliferation. Vol. 6; New York: Cold Spring Harbor Laboratory; 1979: 855–871.

    Google Scholar 

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Use of trade names is for identification only and does not constitute endorsement by the Public Health Service or by the U.S. Department of Health and Human Services.

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Price, P.J., Gregory, E.A. Relationship between in vitro growth promotion and biophysical and biochemical properties of the serum supplement. In Vitro Cell.Dev.Biol.-Plant 18, 576–584 (1982). https://doi.org/10.1007/BF02810081

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  • DOI: https://doi.org/10.1007/BF02810081

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