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Wnt1 and BMP2: two factors recruiting multipotent neural crest progenitors isolated from adult bone marrow

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Abstract

Recent studies have shown that neural crest-derived progenitor cells can be found in diverse mammalian tissues including tissues that were not previously shown to contain neural crest derivatives, such as bone marrow. The identification of those "new" neural crest-derived progenitor cells opens new strategies for developing autologous cell replacement therapies in regenerative medicine. However, their potential use is still a challenge as only few neural crest-derived progenitor cells were found in those new accessible locations. In this study, we developed a protocol, based on wnt1 and BMP2 effects, to enrich neural crest-derived cells from adult bone marrow. Those two factors are known to maintain and stimulate the proliferation of embryonic neural crest stem cells, however, their effects have never been characterized on neural crest cells isolated from adult tissues. Using multiple strategies from microarray to 2D-DIGE proteomic analyses, we characterized those recruited neural crest-derived cells, defining their identity and their differentiating abilities.

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Abbreviations

BMP:

Bone morphogenic proteins

BMSC:

Bone marrow stromal cells

ES:

Embryonic stem cells

GFAP:

Glial fibrillary acidic protein

MSC:

Mesenchymal stem cells

NCC:

Neural crest cells

NCSC:

Neural crest stem cells

NSC:

Neural stem cells

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Acknowledgments

This work was supported by a grant from the Fonds National de la Recherche Scientifique (FNRS) of Belgium, by a grant of the Action de Recherche Concertée de la Communauté Française de Belgique, and by the Belgian League against Multiple Sclerosis associated with Leon Fredericq Foundation. AG is a Marie Curie Host Fellow for Early Stage Research Training, EURON 020589 within the 6th FP of the EU, Marie Curie Actions, Human Resources and Mobility. SWG is a Postdoctoral researcher and PL is a Research Associate of the FNRS. Normalization and data filtering were performed using BRB-ArrayTools software version 3.8.1 developed by Dr. Richard Simons and BRB-ArrayTools Development Team http://linus.nci.nih.gov./BRB-ArrayTools.html.

Author information

Authors and Affiliations

  1. GIGA Neurosciences, University of Liege, Tour de Pathologie 2, CHU Avenue de L’hôpital, 1, 4000, Liège, Belgium

    A. Glejzer, E. Laudet, P. Leprince, B. Rogister & S. Wislet-Gendebien

  2. University of Liege, GIGA Research, Tour GIGA +1, CHU Avenue de L’hôpital, 1, 4000, Liège, Belgium

    B. Hennuy & C. Poulet

  3. Institute of Anatomy, University of Zurich, 8057, Zurich, Switzerland

    O. Shakhova & L. Sommer

  4. GIGA Development, Stem Cells and Regenerative Medicine, University of Liège, Liège, Belgium

    B. Rogister

  5. Department of Neurology, University of Liège, CHU, Liège, Belgium

    B. Rogister

Authors
  1. A. Glejzer
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  2. E. Laudet
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  3. P. Leprince
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  4. B. Hennuy
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  5. C. Poulet
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  6. O. Shakhova
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  7. L. Sommer
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  8. B. Rogister
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  9. S. Wislet-Gendebien
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Corresponding author

Correspondence to S. Wislet-Gendebien.

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Table S1

. Gene expression profile comparison between clone 1 and clone 4. 328 genes have been identified as differentially expressed between clone 1 and 4, with a minimum of 2 fold difference

Table S2

. Gene expression profile comparison between clone 1 and NCSC clone (wnt1-CRE/R26R). 3,578 genes have been identified as differentially expressed between clone 1 and 4, with a minimum of 2 fold difference

Table S3

. Gene expression profile comparison between clone 4 and NCSC clone (wnt1-CRE/R26R). 3966 genes have been identified as differentially expressed between clone 4 and NCSC clone, with a minimum of 2 fold difference

Table S4

. Gene expression profile of specific cell type genes: comparison between clone 1 and clone 4

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Glejzer, A., Laudet, E., Leprince, P. et al. Wnt1 and BMP2: two factors recruiting multipotent neural crest progenitors isolated from adult bone marrow. Cell. Mol. Life Sci. 68, 2101–2114 (2011). https://doi.org/10.1007/s00018-010-0558-5

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  • DOI: https://doi.org/10.1007/s00018-010-0558-5

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