Abstract
In Drosophila, four genes encode for laminin subunits and the formation of two laminin heterotrimers has been postulated. We report the identification of mutations in the Drosophila LamininB2 (LanB2) gene that encodes for the only laminin γ subunit and is found in both heterotrimers. We describe their effects on embryogenesis, in particular the differentiation of visceral tissues with respect to the ECM. Analysis of mesoderm endoderm interaction indicates disrupted basement membranes and defective endoderm migration, which finally interferes with visceral myotube stretching. Extracellular deposition of laminin is blocked due to the loss of the LanB2 subunit, resulting in an abnormal distribution of ECM components. Our data, concerning the different function of both trimers during organogenesis, suggest that these trimers might act in a cumulative way and probably at multiple steps during ECM assembly. We also observed genetic interactions with kon-tiki and thrombospondin, indicating a role for laminin during muscle attachment.
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Acknowledgments
We are grateful to Christian Klämbt for sharing fly stock collections and Susanne Önel for critical reading of the manuscript. We thank Stefan Baumgartner, BDGP, the Bloomington Stock Center, Lynn Cooley, the DGRC, the Developmental Studies Hybridoma Bank, Liselotte Fessler, Michael Hortsch, Elisabeth Knust, Achim Paululat, Renate Renkawitz-Pohl, Rolf Reuter, Maurice Ringuette, John Roote, Martina Schneider, the VDRC, Talila Volk, and Gerd Vorbrüggen for sending materials and fly stocks. This work was supported by the Deutsche Forschungsgemeinschaft to Anne Holz (Ho-2559/3-2 and 3-3).
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Wolfstetter, G., Holz, A. The role of LamininB2 (LanB2) during mesoderm differentiation in Drosophila . Cell. Mol. Life Sci. 69, 267–282 (2012). https://doi.org/10.1007/s00018-011-0652-3
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DOI: https://doi.org/10.1007/s00018-011-0652-3