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Fine mapping of three quantitative trait loci for late blight resistance in tomato using near isogenic lines (NILs) and sub-NILs

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Abstract

An earlier study identified quantitative trait loci (QTLs) lb4, lb5b, and lb11b for quantitative resistance to Phytophthora infestans (late blight) in a backcross population derived from crossing susceptible cultivated tomato (Lycopersicon esculentum) with resistant L. hirsutum. The QTLs were located in intervals spanning 28–47 cM. Subsequently, near-isogenic lines (NILs) were developed for lb4, lb5b, and lb11b by marker-assisted backcrossing to L. esculentum. Sub-NILs containing overlapping L. hirsutum segments across each QTL region were selected and used to validate the QTL effects, fine-map QTLs, and evaluate potential linkage drag between resistance QTLs and QTLs for horticultural traits. The NILs and sub-NILs were evaluated for disease resistance and eight horticultural traits at three field locations. Resistance QTLs were detected in all three sets of NIL lines, confirming the BC1 mapping results. Lb4 mapped near TG609, and between TG182 and CT194, on chromosome 4, a 6.9-cM interval; lb5b mapped to an 8.8-cM interval between TG69a and TG413 on chromosome 5, with the most likely position near TG23; and lb11b mapped to a 15.1-cM interval on chromosome 11 between TG194 and TG400, with the peak centered between CT182 and TG147. Most QTLs for horticultural traits were identified in intervals adjacent to those containing the late blight resistance QTLs. Fine mapping of these QTLs permits the use of marker-assisted selection for the precise introgression of L. hirsutum segments containing late blight resistance alleles separately from those containing deleterious alleles at horticulturally important QTLs.

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Acknowledgements

This research was supported by a USDA-NRI Plant Genome Program, grant 00-35300-9413, to D.A.S. and the California Tomato Commission. The authors thank the USDA station in Salinas, Calif.; Swanton Berry Farm in Majors, Calif; and Jacobs Farm in Swanton, Calif. for field locations and assistance.

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  1. Department of Vegetable Crops, University of California, Davis, CA 95616, USA

    D. J. Brouwer & D. A. St. Clair

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  1. D. J. Brouwer
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  2. D. A. St. Clair
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Correspondence to D. A. St. Clair.

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Communicated by D.B. Neale

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Brouwer, D.J., St. Clair, D.A. Fine mapping of three quantitative trait loci for late blight resistance in tomato using near isogenic lines (NILs) and sub-NILs. Theor Appl Genet 108, 628–638 (2004). https://doi.org/10.1007/s00122-003-1469-8

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