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High-density AFLP map of nonbrittle rachis 1 (btr1) and 2 (btr2) genes in barley (Hordeum vulgare L.)

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An Erratum to this article was published on 13 November 2004

Abstract

Wild relatives of barley disperse their seeds at maturity by means of their brittle rachis. In cultivated barley, brittleness of the rachis was lost during domestication. Nonbrittle rachis of occidental barley lines is controlled by a single gene (btr1) on chromosome 3H. However, nonbrittle rachis of oriental barley lines is controlled by a major gene (btr2) on chromosome 3H and two quantitative trait loci on chromosomes 5HL and 7H. This result suggests multiple mutations of the genes involved in the formation of brittle rachis in oriental lines. The btr1 and btr2 loci did not recombine in the mapping population analyzed. This result agrees with the theory of tight linkage between the two loci. A high-density amplified fragment-length polymorphism (AFLP) map of the btr1/btr2 region was constructed, providing an average density of 0.08 cM/locus. A phylogenetic tree based on the AFLPs showed clear separation of occidental and oriental barley lines. Thus, barley consists of at least two lineages as far as revealed by molecular markers linked to nonbrittle rachis genes.

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Acknowledgements

We thank Dr. T. Konishi (Okayama, Japan) and Drs. K. Takeda and Dr. K. Sato (Okayama University, Kurashiki, Japan) for critically reviewing the manuscript; Dr. A. Paterson (University of Georgia, USA) for comments on the manuscript; Dr. R. von Bothmer (Swedish University of Agricultural Sciences), Dr. B. Salomon (Swedish University of Agricultural Sciences), Dr. B. Gill (Kansas State University, USA), Dr. Y. Ogihara (Yokohama City University, Japan), and Dr. N. Minaka (National Institute of Agro-Environmental Sciences, Tsukuba, Japan) for discussions; and Dr. B. E. Sayed-Tabatabaei (Isfahan University of Technology, Iran) for helping in AFLP analysis. Research grants from Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation to T. Komatsuda are gratefully appreciated. P. Maxim and N. Senthil are STA fellows, and Y. Mano is a research fellow, all supported by JST.

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  1. P. Maxim

    Present address: Research Institute for Cereals and Industrial Crops, Fundulea, 8264, Romania

  2. N. Senthil

    Present address: Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, 641041, India

  3. Y. Mano

    Present address: National Institute of Livestock and Grassland Science, Nishinasuno, 329-2793, Japan

Authors and Affiliations

  1. National Institute of Agrobiological Sciences, Tsukuba, 305-8602, Japan

    T. Komatsuda, P. Maxim, N. Senthil & Y. Mano

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  1. T. Komatsuda
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Correspondence to T. Komatsuda.

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Communicated by J.S. Heslop-Harrison

An erratum to this article can be found at http://dx.doi.org/10.1007/s00122-004-1864-9

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Komatsuda, T., Maxim, P., Senthil, N. et al. High-density AFLP map of nonbrittle rachis 1 (btr1) and 2 (btr2) genes in barley (Hordeum vulgare L.). Theor Appl Genet 109, 986–995 (2004). https://doi.org/10.1007/s00122-004-1710-0

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