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Segregation distortion in Arabidopsis C24/Col-0 and Col-0/C24 recombinant inbred line populations is due to reduced fertility caused by epistatic interaction of two loci

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Abstract

A new large set of reciprocal recombinant inbred lines (RILs) was created between the Arabidopsis accessions Col-0 and C24 for quantitative trait mapping approaches, consisting of 209 Col-0 × C24 and 214 C24 × Col-0 F7 RI lines. Genotyping was performed using 110 evenly distributed framework single nucleotide polymorphism markers, yielding a genetic map of 425.70 cM, with an average interval of 3.87 cM. Segregation distortion (SD) was observed in several genomic regions during the construction of the genetic map. Linkage disequilibrium analysis revealed an association between a distorted region at the bottom of chromosome V and a non-distorted region on chromosome IV. A detailed analysis of the RILs for these two regions showed that an SD occurred when homozygous Col-0 alleles on chromosome IV coincided with homozygous C24 alleles at the bottom of chromosome V. Using nearly isogenic lines segregating for the distorted region we confirmed that this genotypic composition leads to reduced fertility and fitness.

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Acknowledgments

We thank Maik Zehnsdorf, Melanie Lück and Monique Zeh for excellent technical assistance, and Katrin Seehaus and Steffi Zimmermann for plant care. This work was supported by a grants of the Bundesministerium für Bildung und Forschung (BMBF) (#0312275A, #0313061 as part of the GABI program) to T.A., grants of the Deutsche Forschungsgesellschaft (DFG) to T.A. and R.C.M. (AL 387/6-1, 6-2) and to B.K. (ME 931/4-1, 4-2), a grant of the European Community to T.A. (QLG2-CT-2001-01097), and by the Max-Planck-Society.

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Authors and Affiliations

  1. Department of Genetics, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476, Potsdam-Golm, Germany

    Ottó Törjék, Hanna Witucka-Wall, Rhonda C. Meyer, Maria von Korff, Carsten Rautengarten & Thomas Altmann

  2. Max-Planck-Institute of Molecular Plant Physiology, Am Muehlenberg 1, 14476, Golm, Germany

    Thomas Altmann

  3. Institute of Plant Breeding, Seed Science, and Population Genetics, University of Hohenheim, 70593, Stuttgart, Germany

    Barbara Kusterer

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  1. Ottó Törjék
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  2. Hanna Witucka-Wall
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  3. Rhonda C. Meyer
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  4. Maria von Korff
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  5. Barbara Kusterer
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  7. Thomas Altmann
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Corresponding author

Correspondence to Ottó Törjék.

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Communicated by C. Quiros.

Ottó Törjék and Hanna Witucka-Wall contributed equally to this work.

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Törjék, O., Witucka-Wall, H., Meyer, R.C. et al. Segregation distortion in Arabidopsis C24/Col-0 and Col-0/C24 recombinant inbred line populations is due to reduced fertility caused by epistatic interaction of two loci. Theor Appl Genet 113, 1551–1561 (2006). https://doi.org/10.1007/s00122-006-0402-3

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