Skip to main content
SpringerLink
Log in

QTL mapping of yield, agronomic and quality traits in tetraploid potato (Solanum tuberosum subsp. tuberosum)

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Interval mapping of quantitative trait loci (QTL) for 16 yield, agronomic and quality traits in potato was performed on a tetraploid full-sib family comprising 227 clones from a cross between processing clone 12601ab1 and table cultivar Stirling. Thirty-eight AFLP primer combinations and six SSRs provided 514 informative markers which formed a molecular marker map comprising 12 linkage groups (LGs) in 12601ab1 (nine with four homologous chromosomes) which were aligned with 12 in Stirling (11 with four homologous chromosomes), with four partial groups remaining in 12601ab1. Two LGs were identified unequivocally as chromosomes IV and V and eight others were tentatively assigned with chromosomes VII and X unidentified. All of the traits scored had moderately high heritabilities with 54–92% of the variation in clone means over 3 years and two replicates being due to genetic differences. A total of 39 QTLs were identified. A QTL for maturity was identified on chromosome V which explained 56% of the phenotypic variance, whereas the other QTLs individually explained between 5.4 and 16.5%. However, six QTLs were detected for after-cooking blackening and four for each of regularity of tuber shape, fry colour both after storage at 4 and 10°C and sprouting. Just two QTLs were found for each of yield, the two ‘overall’ scores, crop emergence, tuber size and common scab and just one QTL was detected for each of dry matter content, keeping quality, growth cracks and internal condition. The implications for practical potato breeding and for practical linkage and QTL analysis in autotetraploids are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Bonierbale MW, Plaisted RL, Tanksley SD (1993) A test of the maximum heterozygosity hypothesis using molecular markers in tetraploid potatoes. Theor Appl Genet 86:481–491

    Article  CAS  Google Scholar 

  • Bradshaw JE, Stewart HE, Wastie RL, Dale MFB, Phillips MS (1995) Use of seedling progeny tests for genetical studies as part of a potato (Solanum tuberosum subsp. tuberosum) breeding programme. Theor Appl Genet 90:899–905

    Article  Google Scholar 

  • Bradshaw JE, Dale MFB, Mackay GR (2003) Use of mid-parent values and progeny tests to increase the efficiency of potato breeding for combined processing quality and disease and pest resistance. Theor Appl Genet 107:36–42

    PubMed  CAS  Google Scholar 

  • Bradshaw JE, Pande B, Bryan GJ, Hackett CA, McLean K, Stewart HE, Waugh R (2004) Interval mapping of quantitative trait loci for resistance to late blight [Phytophthora infestans (Mont.) de Bary], height and maturity in a tetraploid population of potato (Solanum tuberosum subsp. tuberosum). Genetics 168:983–995

    Article  PubMed  CAS  Google Scholar 

  • Brown J, Mackay GR, Bain H, Griffith DW, Allison MJ (1990) The processing potential of tubers of the cultivated potato, Solanum tuberosum L., after storage at low temperatures. 2. Sugar concentration. Pot Res 33:219–227

    Article  Google Scholar 

  • Bryan GJ, McLean K, Bradshaw JE, De Jong WS, Phillips M, Castelli L, Waugh R (2002) Mapping QTLs for resistance to the cyst nematode Globodera pallida derived from the wild potato species Solanum vernei. Theor Appl Genet 105:68–77

    Article  PubMed  CAS  Google Scholar 

  • Bryan GJ, McLean K, Pande B, Purvis A, Hackett CA, Bradshaw JE, Waugh R (2004) Genetical dissection of H3-mediated polygenic PCN resistance in a heterozygous autotetraploid potato population. Mol Breed 14:105–116

    Article  CAS  Google Scholar 

  • Celebi-Toprak F, Watanabe JA, Watanabe KN (2005) Molecular markers in identification of genotypic variation. In: Razdan MK, Mattoo AK (eds) Genetic improvement of Solanaceous crops: potato, vol 1. Science Publishers, Enfield, pp 115–141

    Google Scholar 

  • Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971

    PubMed  CAS  Google Scholar 

  • Dong F, Song S, Naess SK, Helgeson JP, Gebhardt C, Jiang J (2000) Development and applications of a set of chromosome-specific cytogenetic DNA markers in potato. Theor Appl Genet 101:1001–1007

    Article  CAS  Google Scholar 

  • Freyre R, Douches DS (1994) Development of a model for marker-assisted selection of specific gravity in diploid potato across environments. Crop Sci 34:1361–1368

    Article  Google Scholar 

  • Freyre R, Warnke S, Sosinski B, Douches DS (1994) Quantitative trait locus analysis of tuber dormancy in diploid potato (Solanum spp.). Theor Appl Genet 89:474–480

    Article  CAS  Google Scholar 

  • GENSTAT 5 Committee (1993) GENSTAT 5 release 3 reference manual. Clarendon Press, Oxford

    Google Scholar 

  • Ghislain M, Spooner DM, Rodriguez F, Villamon F, Nunez J, Vasquez C, Waugh R, Bonierbale M (2004) Selection of highly informative and user-friendly microsatellites (SSRs) for genotyping of cultivated potato. Theor Appl Genet 108:881–890

    Article  PubMed  CAS  Google Scholar 

  • Hackett CA, Bradshaw JE, Meyer RC, McNicol JW, Milbourne D, Waugh R (1998) Linkage analysis in tetraploid species: a simulation study. Genet Res Camb 71:143–154

    CAS  Google Scholar 

  • Hackett CA, Bradshaw JE, McNicol JW (2001) Interval mapping of QTLs in autotetraploid species. Genetics 159:1819–1832

    PubMed  CAS  Google Scholar 

  • Hackett CA, Pande B, Bryan GJ (2003) Constructing linkage maps in autotetraploid species using simulated annealing. Theor Appl Genet 106:1107–1115

    PubMed  CAS  Google Scholar 

  • Hackett CA, Luo ZW (2003) TetraploidMap: construction of a linkage map in autotetraploid species. J Hered 94:358–359

    Article  PubMed  CAS  Google Scholar 

  • Li XQ, De Jong H, De Jong DM, De Jong WS (2005) Inheritance and genetic mapping of tuber eye depth in cultivated diploid potatoes. Theor Appl Genet 110:1068–1073

    Article  PubMed  CAS  Google Scholar 

  • Luo ZW, Hackett CA, Bradshaw JE, McNicol JW, Milbourne DM (2000) Predicting parental genotypes and gene segregation for tetrasomic inheritance. Theor Appl Genet 100:1067–1073

    Article  Google Scholar 

  • Luo ZW, Hackett CA, Bradshaw JE, McNicol JW, Milbourne DM (2001) Construction of a genetic linkage map in tetraploid species using molecular markers. Genetics 157:1369–1385

    PubMed  CAS  Google Scholar 

  • Mackay GR (2005) Propagation by traditional breeding methods. In: Razdan MK, Mattoo AK (eds) Genetic improvement of Solanaceous crops: potato, vol 1. Science Publishers, Enfield, pp 65–81

    Google Scholar 

  • Mackay GR, Dale MFB (1990) Quality in potatoes. SCRI Annual Report 1990, pp 9–12

  • Menendez CM, Ritter E, Schäfer-Pregl R, Walkemeier B, Kalde A, Salamini F, Gebhardt C (2002) Cold sweetening in diploid potato: mapping quantitative trait loci and candidate genes. Genetics 162:1423–1434

    PubMed  CAS  Google Scholar 

  • Meyer RC, Milbourne DM, Hackett CA, Bradshaw JE, McNicol JW, Waugh R (1998) Linkage analysis in tetraploid potato and association of markers with quantitative resistance to late blight (Phytophthora infestans). Mol Gen Genet 259:150–160

    Article  PubMed  CAS  Google Scholar 

  • Rouppe van der Voort JNAM, van Zandvoort P, van Eck HJ, Folkertsma RT, Hutten RCB, Draaistra J, Gommers FJ, Jacobsen E, Helder J, Bakker J (1997) Use of allele specificity of comigrating AFLP markers to align genetic maps from different potato genotypes. Mol Gen Genet 255:438–447

    Article  Google Scholar 

  • Schäfer-Pregl R, Ritter E, Concilio L, Hesselbach J, Lovatti L, Walkemeier B, Thelen H, Salamini F, Gebhardt C (1998) Analysis of quantitative trait loci (QTLs) and quantitative trait alleles (QTAs) for potato tuber yield and starch content. Theor Appl Genet 97:834–846

    Article  Google Scholar 

  • Stam P (1993) Construction of integrated genetic linkage maps by means of a new computer package: Joinmap. Plant J 3:739–744

    Article  CAS  Google Scholar 

  • Swaminathan MS, Howard HW (1953) The cytology and genetics of the potato (Solanum tuberosum) and related species. Bibliogr Genet 16:1–192

    Google Scholar 

  • Van den Berg JH, Ewing EE, Plaisted RL, McMurry S, Bonierbale MW (1996a) QTL analysis of potato tuberization. Theor Appl Genet 93:307–316

    Article  Google Scholar 

  • Van den Berg JH, Ewing EE, Plaisted RL, McMurry S, Bonierbale MW (1996b) QTL analysis of potato tuber dormancy. Theor Appl Genet 93:317–324

    Article  Google Scholar 

  • Van Eck HJ, Jacobs JME, van Dijk J, Stiekema WJ, Jacobsen E (1993) Identification and mapping of three flower colour loci of potato (S. tuberosum L.) by RFLP analysis. Theor Appl Genet 86:295–300

    Article  Google Scholar 

  • Van Eck HJ, Jacobs JME, Stam P, Ton J, Stiekema WJ, Jacobsen E (1994) Multiple alleles for tuber shape in diploid potato detected by qualitative and quantitative genetic analysis using RFLPs. Genetics 137:303–309

    PubMed  Google Scholar 

  • Von Scheele C, Svensson G, Rasmussen J (1937) Die bestimmung des starkegehalts und der trockensubstanz der kartoffel mit hilfe des spezifischen gewichts. Landw Vers Sta 127:67–96

    Google Scholar 

Download references

Acknowledgements

Thanks are due to Margaret McInroy, Karen McLean, Drummond Todd and Ralph Wilson for technical support, Jim McNicol for statistical advice, and to the British Potato Council (Ph.D. studentship to B.P.), the Scottish Executive Environment and Rural Affairs Department and the United Kingdom Biotechnology and Biological Sciences Research Council (GAIT Project) for funding. Dr. Zewei Luo, now at the University of Birmingham, developed the theory and software for linkage analysis whilst on the GAIT project.

Author information

Authors and Affiliations

  1. SCRI, Invergowrie, Dundee, DD2 5DA, UK

    John E. Bradshaw, Barnaly Pande, Robbie Waugh & Glenn J. Bryan

  2. Biomathematics and Statistics Scotland, SCRI, Invergowrie, Dundee, DD2 5DA, UK

    Christine A. Hackett

Authors
  1. John E. Bradshaw
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Christine A. Hackett
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Barnaly Pande
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Robbie Waugh
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Glenn J. Bryan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to John E. Bradshaw.

Additional information

Communicated by M. Kearsey.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bradshaw, J.E., Hackett, C.A., Pande, B. et al. QTL mapping of yield, agronomic and quality traits in tetraploid potato (Solanum tuberosum subsp. tuberosum). Theor Appl Genet 116, 193–211 (2008). https://doi.org/10.1007/s00122-007-0659-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-007-0659-1

Keywords

Search

Navigation