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Fine-mapping of the woolly gene controlling multicellular trichome formation and embryonic development in tomato

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Abstract

Trichomes are small hairs that originate from the epidermal cells of nearly all land plants, and they exist in unicellular and multicellular forms. The regulatory pathway of unicellular trichomes in Arabidopsis is well characterized. However, little is known about the multicellular trichome formation in tomato (Solanum lycopersicum). The woolly (Wo) gene controls multicellular trichome initiation and leads to embryonic lethality when homozygous in tomato. To clone and characterize Wo, the gene was fine-mapped to a DNA fragment of ~200 kb using the map-based cloning strategy. A series of sequence-based molecular markers, including simple sequence repeat, sequence characterized amplified region, and cleaved amplified polymorphic sequence were utilized in this study. Analysis of the sequence indicated that this region carries 19 putative open reading frames. These results will provide not only the important information for the isolation and characterization of Wo but also the starting point for studying the regulatory pathway responsible for trichome formation and embryonic lethality in tomato.

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Acknowledgments

The authors are grateful to Dr. G. P. Wang from the South China Agricultural University and Dr. H. H. Kuang from our College for the helpful advice. The authors acknowledge the Tomato Genetics Resource Center, University of California, USA, for providing the seed stocks of the introgression lines and the woolly mutant LA3186. This project was supported by grants from the 973 project (No. 2011CB100600), the National Natural Science Foundation of China (Nos. 30971997 and 30921002) and the Research Fund for the Doctoral Program of Higher Education of China (4010-081023).

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China

    Changxian Yang, Junhong Zhang & Zhibiao Ye

  2. Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China

    Hanxia Li, Taotao Wang & Zhibiao Ye

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  1. Changxian Yang
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  2. Hanxia Li
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  3. Junhong Zhang
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Correspondence to Zhibiao Ye.

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Communicated by M. Havey.

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Yang, C., Li, H., Zhang, J. et al. Fine-mapping of the woolly gene controlling multicellular trichome formation and embryonic development in tomato. Theor Appl Genet 123, 625–633 (2011). https://doi.org/10.1007/s00122-011-1612-x

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  • DOI: https://doi.org/10.1007/s00122-011-1612-x

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