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Identification of a new soybean rust resistance gene in PI 567102B

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Abstract

Soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. and P. Syd. is one of the most economically important diseases of soybean (Glycine max (L.) Merr.). Durable resistance to P. pachyrhizi is the most effective long-term strategy to control SBR. The objective of this study was to investigate the genetics of resistance to P. pachyrhizi in soybean accession PI 567102B. This accession was previously identified as resistant to SBR in Paraguay and to P. pachyrhizi isolates from seven states in the USA (Alabama, Florida, Georgia, Louisiana, Mississippi, South Carolina, and Texas). Analysis of two independent populations, one in which F2 phenotypes were inferred from F2-derived F3 (F2:3) families and the other in which F2 plants had phenotypes measured directly, showed that the resistance in PI 567102B was controlled by a single dominant gene. Two different isolates (MS06-1 and LA04-1) at different locations (Stoneville, MS and Ft. Detrick, MD) were used to independently assay the two populations. Linkage analysis of both populations indicated that the resistance locus was located on chromosome 18 (formerly linkage group G), but at a different location than either Rpp1 or Rpp4, which were previously mapped to this linkage group. Therefore, the SBR resistance in PI 567102B appeared to be conditioned by a previously unreported locus, with an underlying single dominant gene inferred. We propose this gene to be designated Rpp6. Incorporating Rpp6 into improved soybean cultivars may have wide benefits as PI 567102B has been shown to provide resistance to P. pachyrhizi isolates from Paraguay and the US.

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Acknowledgments

The authors appreciate the dedicated work of the USDA-ARS personnel at Stoneville, MS, including Ms. Avis Clark, Mr. Kenneth Courtney, Ms. Loria Courtney, Ms. Angelie Davis, Mr. Wilson Davis, Mr. Philip Handly, Mr. Hans Hinrichsen, Mr. Ronnie Griffin, Mr. Matt Kersh, Mr. Jai McDonnieal, Mr. Marcus Porter, Mr. Charles Rucker; and Ms. Rebecca Simpson. Seed production in Puerto Rico by Dr. Ricardo Goenaga and Mr. Carlos Torres of the USDA-ARS Tropical Agriculture Research Station was critical for the timely completion of this project. The authors also appreciate the efforts of Dr. Lawrence Young to establish a laboratory in the USDA-ARS Stoneville Research Quarantine Facility for soybean rust research and to Dr. David Walker for sharing his unpublished screening data. This research could not have been accomplished without these combined efforts. This research was partially supported by the USDA-ARS projects 6402-21220-010-00 and 1920-22000-035-00D. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer.

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Authors and Affiliations

  1. USDA-ARS, Crop Genetics Research Unit, Stoneville, MS, 38776, USA

    Shuxian Li, James R. Smith & Jeffery D. Ray

  2. USDA-ARS, Foreign Disease-Weed Science Research Unit, Ft. Detrick, MD, 21702, USA

    Reid D. Frederick

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  1. Shuxian Li
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Correspondence to Shuxian Li.

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Communicated by B. Diers.

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Li, S., Smith, J.R., Ray, J.D. et al. Identification of a new soybean rust resistance gene in PI 567102B. Theor Appl Genet 125, 133–142 (2012). https://doi.org/10.1007/s00122-012-1821-y

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