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Inhibition of the Na/Bicarbonate Cotransporter NBCe1-A by diBAC Oxonol Dyes Relative to Niflumic Acid and a Stilbene

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Notes

  1. In the diBA(5)C4 study, two of the six (Norm I NBC)−1 values obtained at 20 μM diBAC + 40 μM DIDS were not included in our analysis because they appeared to be outliers. These two values (8.63 and 9.91) had a mean (Norm I NBC)−1 of 9.27, which was 2.3-fold larger than the mean value of 3.96 obtained from the other four experiments.

  2. Approximately ∼7 mM HCO 3 and 20-30 mM Na+ are the apparent K M values of NBCe1-A expressed in oocytes (Sciortino & Romero, 1999; Grichtchenko, Romero & Boron, 2000; McAlear et al., 2006).

References

  • Alper SL, Chernova MN, Williams J, Zasloff M, Law F-Y, Knauf PA (1998) Differential inhibition of AE1 and AE2 anion exchangers by oxonol dyes and by novel polyaminosterol analogs of the shark antibiotic squalamine. Biochem Cell Biol 76:799–806

    Article  PubMed  CAS  Google Scholar 

  • Belachgar F, Hulin P, Anagnostopoulos T, Planelles G (1994) Triflocin, a novel inhibitor for the Na-HCO3 symport in the proximal tubule. Br J Pharmacol 112:465–470

    PubMed  CAS  Google Scholar 

  • Bevensee MO, Schmitt BM, Choi I, Romero MF, Boron WF (2000) An electrogenic Na+- HCO 3 cotransporter (NBC) with a novel COOH terminus, cloned from rat brain. Am J Physiol Cell Physiol 278:1200–1211

    Google Scholar 

  • Cabantchik ZI, Greger R (1992) Chemical probes for anion transporters of mammalian cell membranes. Am J Physiol Cell Physiol 262:803–827

    Google Scholar 

  • Cousin JL, Motais R (1979) Inhibition of anion permeability by amphiphilic compounds in human red cell: evidence for an interaction of niflumic acid with the Band 3 protein. J Membr Biol 46:125–153

    Article  PubMed  CAS  Google Scholar 

  • Ducoudret O, Diakov A, Müller-Berger S, Romero MF, Frömter E (2001) The renal Na- HCO 3 cotransporter expressed in Xenopus laevis oocytes: inhibition by tenidap and benzamil and effect of temperature on transport rate and stoichiometry. Pfluegers Arch 442:709–717

    Article  CAS  Google Scholar 

  • DeFelice LJ (2004) Transporter structure and mechanism. Trends Neurosci 27:352–359

    Article  PubMed  CAS  Google Scholar 

  • Fröhlich O, Gunn RB (1987) Interactions of inhibitors on anion transporter of human erythrocyte. Am J Physiol Cell Physiol 252:153–162

    Google Scholar 

  • Grichtchenko II, Romero MF, Boron WF (2000) Extracellular HCO 3 dependence of electrogenic Na/HCO3 cotransporters cloned from salamander and rat kidney. J Gen Physiol 115:533–545

    Article  PubMed  CAS  Google Scholar 

  • Haugland RP (2005) The Handbook. A Guide to Fluorescent Probes and Labeling Technologies, 10th ed. Carlsbad, CA: Invitrogen, pp 983–984

    Google Scholar 

  • Kaback HR, Dunten R, Frillingos S, Venkatesan P, Kwaw I, Zhang W, Ermolova N (2007) Site-directed alkylation and the alternating access model for LacY. Proc Natl Acad Sci USA 104:491–494

    Article  PubMed  CAS  Google Scholar 

  • Khandoudi N, Albadine J, Robert P, Krief S, Berrebi-Bertrand I, Martin X, Bevensee MO, Boron WF, Bril A (2001) Inhibition of the cardiac electrogenic sodium bicarbonate cotransporter reduces ischemic injury. Cardiovasc Res 52:387–396

    Article  PubMed  CAS  Google Scholar 

  • Knauf PA, Law F-Y, Hahn K (1995) An oxonol dye is the most potent known inhibitor of band 3-mediated anion exchange. Am J Physiol Cell Physiol 269:1073–1077

    Google Scholar 

  • Knauf PA, Law F-Y, Vivian Leung T-W, Gehret AU, Perez ML (2002) Substrate-dependent reversal of anion transport site orientation in the human red blood cell anion-exchange protein, AE1. Proc Natl Acad Sci USA 99:10861–10864

    Article  PubMed  CAS  Google Scholar 

  • Knauf PA, Mann NA (1984) Use of niflumic acid to determine the nature of the asymmetry of the human erythrocyte anion exchange system. J Gen Physiol 83:703–725

    Article  PubMed  CAS  Google Scholar 

  • Knauf PA, Raha NM, Spinelli LJ (2000) The noncompetitive inhibitor WW781 senses changes in erythrocyte anion exchanger (AE1) transport site conformation and substrate binding. J Gen Physiol 115:159–173

    Article  PubMed  CAS  Google Scholar 

  • Lorenz C, Pusch M, Jentsch TJ (1996) Heteromultimeric ClC chloride channels with novel properties. Proc Natl Acad Sci USA 93:13362–13366

    Article  PubMed  CAS  Google Scholar 

  • Lu J, Boron WF (2007) Reversible and irreversible interactions of DIDS with the human electrogenic Na/HCO3 cotransporter NBCe1-A: role of lysines in the KKMIK motif of TM5. Am J Physiol Cell Physiol 292:C1787–C1798

    Article  PubMed  CAS  Google Scholar 

  • McAlear SD, Liu X, Williams JB, McNicholas-Bevensee CM, Bevensee MO (2006) Electrogenic Na/HCO3 cotransporter (NBCe1) variants expressed in Xenopus oocytes: functional comparison and roles of the amino and carboxy termini. J Gen Physiol 127:639–658

    Article  PubMed  CAS  Google Scholar 

  • Raha NM, Spinelli LJ, Knauf PA (1993) WW781, a potent reversible inhibitor of red cell Cl flux, binds to band 3 by a two-step mechanism. Am J Physiol Cell Physiol 265:C521–C532

    CAS  Google Scholar 

  • Romero MF, Fong P, Berger UV, Hediger MA, Boron WF (1998) Cloning and functional expression of rNBC, an electrogenic Na+- HCO 3 cotransporter from rat kidney. Am J Physiol Renal Physiol 274:425–432

    Google Scholar 

  • Romero MF, Fulton CM, Boron WF (2004) The SLC4 family of HCO 3 transporters. Pfluegers Arch 447:495–509

    Article  CAS  Google Scholar 

  • Romero MF, Hediger MA, Boulpaep EL, Boron WF (1997) Expression cloning and characterization of a renal electrogenic Na+/HCO 3 cotransporter. Nature 387:409–413

    Article  PubMed  CAS  Google Scholar 

  • Roos A, Boron WF (1981) Intracellular pH. Physiol Rev 61:296–434

    PubMed  CAS  Google Scholar 

  • Sciortino CM, Romero MF (1999) Cation and voltage dependence of rat kidney electrogenic Na+-HCO 3 cotransporter, rkNBC, expressed in oocytes. Am J Physiol Renal Physiol 277:611–623

    Google Scholar 

  • Zhao J, Zhou Y, Boron WF (2003) Effect of isolated removal of either basolateral HCO 3 or basolateral CO2 on HCO 3 reabsorption by rabbit S2 proximal tubule. Am J Physiol Renal Physiol 285:359–369

    Google Scholar 

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Acknowledgements

This work was supported by the American Heart Association Southeast Affiliate (0265083B) and the National Institutes of Health/National Institute of Neurological Disorders and Stroke (NS046653). We thank Dr. Carmel M. McNicholas-Bevensee for assisting in the statistical analyses as well as reading the manuscript and providing helpful comments and suggestions.

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Correspondence to Mark O. Bevensee.

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Liu, X., Williams, J.B., Sumpter, B.R. et al. Inhibition of the Na/Bicarbonate Cotransporter NBCe1-A by diBAC Oxonol Dyes Relative to Niflumic Acid and a Stilbene. J Membrane Biol 215, 195–204 (2007). https://doi.org/10.1007/s00232-007-9018-z

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