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Soil Water Content and Organic Carbon Availability Are Major Determinants of Soil Microbial Community Composition

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Abstract

Exploration of environmental factors governing soil microbial community composition is long overdue and now possible with improved methods for characterizing microbial communities. Previously, we observed that rice soil microbial communities were distinctly different from tomato soil microbial communities, despite management and seasonal variations within soil type. Potential contributing factors included types and amounts of organic inputs, organic carbon content, and timing and amounts of water inputs. Of these, both soil water content and organic carbon availability were highly correlated with observed differences in composition. We examined how organic carbon amendment (compost, vetch, or no amendment) and water additions (from air dry to flooded) affect microbial community composition. Using canonical correspondence analysis of phospholipid fatty acid data, we determined flooded, carbon-amended (+C) microcosm samples were distinctly different from other +C samples and unamended (–C) samples. Although flooding without organic carbon addition influenced composition some, organic carbon addition was necessary to substantially alter community composition. Organic carbon availability had the same general effects on microbial communities regardless of whether it was compost or vetch in origin. In addition, flooded samples, regardless of organic carbon inputs, had significantly lower ratios of fungal to bacterial biomarkers, whereas under drier conditions and increased organic carbon availability the microbial communities had higher proportions of fungal biomass. When comparing field and microcosm soil, flooded +C microcosm samples were most similar to field-collected rice soil, whereas all other treatments were more similar to field-collected tomato soil. Overall, manipulating water and carbon content selected for microbial communities similar to those observed when the same factors were manipulated at the field scale.

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Acknowledgments

The authors thank the U.S. Environmental Protection Agency’s Center for Ecological Health Research at U.C. Davis, the NIEHS Superfund Basic Research Program (2P42 ESO4699), and a grant from the Kearney Foundation of Soil Science. The comments of K.M. Batten, K.A. Hicks, and three anonymous reviewers significantly improved the manuscript.

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  1. Department of Land, Air and Water Resources, University of California, Davis, One Shields Avenue, Davis, CA, 95616-8627, USA

    R.E. Drenovsky, D. Vo, K.J. Graham & K.M. Scow

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  1. R.E. Drenovsky
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Correspondence to R.E. Drenovsky.

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Drenovsky, R., Vo, D., Graham, K. et al. Soil Water Content and Organic Carbon Availability Are Major Determinants of Soil Microbial Community Composition. Microb Ecol 48, 424–430 (2004). https://doi.org/10.1007/s00248-003-1063-2

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