Skip to main content
SpringerLink
Log in

Limnothrix redekei (Van Goor) Meffert (Cyanobacteria) Strains from Lake Kastoria, Greece Form a Separate Phylogenetic Group

  • Published:
Microbial Ecology Aims and scope Submit manuscript

Abstract

Three strains of Limnothrix (Cyanobacteria) isolated from Lake Kastoria, Greece, were characterized based on their morphological features and 16S rRNA gene sequences. The Limnothrix isolates 007a, 165a, and 165c can morphologically be assigned to Limnothrix redekei (Van Goor) Meffert. The 16S rRNA gene of the Limnothrix strains showed a 99% similarity to the 16S rRNA gene of Planktothrix sp. FP1. Limnothrix redekei strains 165a, 165c, 007a and Planktothrix sp. FP1 formed a separate cluster in the cyanobacterial 16S rRNA gene tree. It was distinct from the Pseudanabaena cluster, which included the other Limnothrix strains isolated from northern temperate lakes. This is the first report on the phylogeny of L. redekei strains originating from a Mediterranean lake (southern Europe) and provides new data about the genus Limnothrix.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1
Figure 2

Similar content being viewed by others

References

  1. K Anagnostidis J Komárek (1988) ArticleTitleModern approach to the classification system of cyanophytes. 3—Oscillatoriales. Arch Hydrobiol Suppl 80 327–472

    Google Scholar 

  2. AE Bailey-Watts (1972) ArticleTitleThe algal plankton of Loch Leven, Kinross Proc Roy Soc Edinburgh Sect B 74 135–156

    Google Scholar 

  3. RW Castenholz R Rippka M Herdman A Wilmotte (2001) Form-genus VI. Limnothrix Meffert 1988. DR Boone RW Castenholz (Eds) Bergey’s Manual of Systematic Bacteriology, vol. 1 Springer-Verlag New York 546

    Google Scholar 

  4. RW Castenholz R Rippka M Herdman A Wilmotte (2001) Subsection III. (Formerly Oscillatoriales Elenkin 1934). DR Boone RW Castenholz (Eds) Bergey’s Manual of Systematic Bacteriology, vol. 1 Springer-Verlag New York 539–562

    Google Scholar 

  5. NM de Chazal S Smaglinski GD Smith (1992) ArticleTitleMethods involving light variation of cyanobacteria: characterization of isolates from central Australia. Appl Environ Microbiol 58 3561–3566

    Google Scholar 

  6. U Edwards T Rogall H Blöcker M Emde EC Böttger (1989) ArticleTitleIsolation and direct complete nucleotide determination of entire genes. Characterisation of a gene coding for 16S ribosomal RNA. Nucleic Acids Res 17 7843–7853 Occurrence Handle1:CAS:528:DyaK3cXjtFKksg%3D%3D Occurrence Handle2798131

    CAS  PubMed  Google Scholar 

  7. P Fay (1983) The Blue-greens (Cyanophyta, Cyanobacteria). Studies in Biology/Institute of Biology, no. 160 Edward Arnold Publishers Southampton, UK 17–18

    Google Scholar 

  8. CE Gibson RB Wood EL Dickson DH Jewson (1971) ArticleTitleThe succession of phytoplankton Lough Neagh 1968–1970. Mitt Int Ver Theor Angew Limnol 19 146–160

    Google Scholar 

  9. TA Hall (1999) ArticleTitleBioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41 95–98 Occurrence Handle1:CAS:528:DC%2BD3cXhtVyjs7Y%3D

    CAS  Google Scholar 

  10. M Hernández-Mariné (1996) ArticleTitleElectron microscopic characterization of Microcoleus chthonoplastes THUR. (Cyanobacteria). Algolog Studies 83 347–365

    Google Scholar 

  11. F Hindák MT Moustaka (1988) ArticleTitlePlanktic cyanophytes of Lake Volvi, Greece. Arch Hydrobiol Suppl 80 497–528

    Google Scholar 

  12. W Hönerlage D Hahn J Zeyer (1995) ArticleTitleDetection of mRNA of nprM in Bacillus megaterium ATCC 14581 grown in soil by whole-cell hybridization. Arch Microbiol 163 235–241

    Google Scholar 

  13. J Köhler S Hoeg (2000) ArticleTitlePhytoplankton selection in a river–lake system during two decades of changing nutrient supply. Hydrobiologia 424 13–24 Occurrence Handle10.1023/A:1003988508599

    Article  Google Scholar 

  14. J Komárek MTP Azevedo (2000) ArticleTitleGeitlerinema unigranulatum, a common tropical cyanoprokaryote from freshwater reservoirs in Brazil. Algolog Studies 99 39–52

    Google Scholar 

  15. MJ Laamanen MF Gugger JM Lehtimäki K Haukka K Sivonen (2001) ArticleTitleDiversity of toxic and non-toxic Nodularia isolates (cyanobacteria) and filaments from the Baltic sea. Appl Environ Microbiol 67 4638–4647 Occurrence Handle10.1128/AEM.67.10.4638-4647.2001 Occurrence Handle1:CAS:528:DC%2BD3MXns1Wisbk%3D Occurrence Handle11571167

    Article  CAS  PubMed  Google Scholar 

  16. J Lehtimäki C Lyra S Suomalainen P Sundman L Rouhiainen L Paulin M Salkinoja-Salonen K Sivonen (2000) ArticleTitleCharacterization of Nodularia strains, cyanobacteria from brackish waters, by genotypic and phenotypic methods. Int J Syst Evol Microbiol 50 1043–1053 Occurrence Handle10843044

    PubMed  Google Scholar 

  17. C Lepère A Wilmotte B Meyer (2000) ArticleTitleMolecular diversity of Microcystis strains (Cyanophyaceae, Chroococales) based on 16S rDNA sequences. Syst Geogr Pl 70 275–283

    Google Scholar 

  18. C Lyra S Suomalainen M Gugger C Vézie P Sundman L Paulin K Sivonen (2001) ArticleTitleMolecular characterization of planktic cyanobacteria of Anabaena, Aphanizomenon, Microcystis and Planktothrix genera. Int J Syst Evol Microbiol 51 513–526 Occurrence Handle1:CAS:528:DC%2BD3MXivVOrurw%3D Occurrence Handle11321098

    CAS  PubMed  Google Scholar 

  19. J Mayer MT Dokulil M Salbrechter M Berger T Posch G Pfister AKT Kirschner B Velimirov A Steitz T Ulbricht (1997) ArticleTitleSeasonal successions and trophic relations between phytoplankton, zooplankton, ciliate and bacteria in a hypertrophic shallow lake in Vienna, Austria. Hydrobiologia 342 IssueID343 165–174 Occurrence Handle10.1023/A:1017098131238

    Article  Google Scholar 

  20. ME Meffert (1987) ArticleTitlePlanktic unsheathed filaments (Cyanophyceae) with polar and central gas-vacuoles. I. Their morphology and taxonomy. Arch Hydrobiol Suppl 76 315–346

    Google Scholar 

  21. ME Meffert (1988) ArticleTitleLimnothrix Meffert nov. gen. The unsheathed planktic cyanophycean filaments with polar and central gas-vacuoles. Arch Hydrobiol Suppl 80 269–276

    Google Scholar 

  22. ME Meffert (1989) ArticleTitlePlanktic unsheathed filaments (Cyanophyceae) with polar and central gas-vacuoles. II. Biology population dynamics and biotopes of Limnothrix redekei (Van Goor) Meffert. Arch Hydrobiol 116 257–282

    Google Scholar 

  23. ME Meffert (1989) ArticleTitleIn situ and in vitro photosynthesis of Limnothrix redekei (Cyanophyta). Arch Hydrobiol 119 299–313

    Google Scholar 

  24. ME Meffert R Oberhäuser J Overbeck (1981) ArticleTitleMorphology and taxonomy of Oscillatoria redekei (cyanophyta). Br Phycol J 16 107–114

    Google Scholar 

  25. M Moustaka-Gouni (1993) ArticleTitlePhytoplankton succession and diversity in a warm monomictic, relatively shallow lake: Lake Volvi, Macedonia, Greece. Hydrobiologia 249 33–42

    Google Scholar 

  26. LR Mur OM Skulberg H Utkilen (1999) Cyanobacteria in the environment. I Chorus J Bartram (Eds) Toxic Cyanobacteria in Water World Health Organization, E & FN Spon London 179–209

    Google Scholar 

  27. B Nixdorf (1994) ArticleTitlePolymixis of a shallow lake (Grosser Müggelsee, Berlin) and its influence on seasonal phytoplankton dynamics. Hydrobiologia 176 173–186

    Google Scholar 

  28. KA Palinska W Liesack E Rhiel WE Krumbein (1996) ArticleTitlePhenotype variability of identical genotypes: the need for a combined approach in cyanobacterial taxonomy demonstrated on Merismopedia-like isolates. Arch Microbiol 166 224–233 Occurrence Handle10.1007/s002030050378 Occurrence Handle1:CAS:528:DyaK28XmsVKhtb0%3D Occurrence Handle8824145

    Article  CAS  PubMed  Google Scholar 

  29. F Pomati S Sacchi C Rossetti S Giovannardi H Onodera Y Oshima (2000) ArticleTitleThe freshwater cyanobacterium Plankothrix sp. FP1: molecular identification and detection of paralytic shellfish poisoning toxins. J Phycol 36 553–562 Occurrence Handle10.1046/j.1529-8817.2000.99181.x Occurrence Handle1:CAS:528:DC%2BD3cXmslarsbY%3D

    Article  CAS  Google Scholar 

  30. CS Reynolds (1994) ArticleTitleThe long, the short and the stalled: on the attributes of phytoplankton selected by physical mixing in lakes and rivers. Hydrobiologia 189 9–21 Occurrence Handle10.1007/BF00007405

    Article  Google Scholar 

  31. R Rippka (1988) ArticleTitleIsolation and purification of cyanobacteria. Methods Enzymol 167 3–27 Occurrence Handle1:CAS:528:DyaL1MXit1yjur0%3D Occurrence Handle3148836

    CAS  PubMed  Google Scholar 

  32. C Rojo M Alvarez Cobelas (1994) ArticleTitlePopulation dynamics of Limnothrix redekei, Oscillatoria lanceaformis, Planktothrix agardhii and Pseudanabaena limnetica (cyanobacteria) in a shallow hypertrophic lake (Spain). Hydrobiologia 276 165–171

    Google Scholar 

  33. J Rücker C Wiedner P Zippel (1997) ArticleTitleFactors controlling the dominance of Planktothrix agardhii and Limnothrix redekei in eutrophic shallow lakes. Hydrobiologia 342/343 107–115

    Google Scholar 

  34. N Saitou M Nei (1987) ArticleTitleThe neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406–425 Occurrence Handle1:STN:280:BieC1cbgtVY%3D Occurrence Handle3447015

    CAS  PubMed  Google Scholar 

  35. SMF Silva RN Pienaar (2000) Benthic marine cyanophyceae from Kwa-Zulu Natal, South Africa. L Lies R Schnetter (Eds) Bibliotheca Phycologica . Berlin-Stuttgart 231–236

    Google Scholar 

  36. E Stackebrandt BM Goebel (1994) ArticleTitleTaxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44 486–849

    Google Scholar 

  37. S Suda MM Watanabe S Otsuka A Mahakahant W Yongmanitchai N Nopartnaraporn Y Liu JG Day (2002) ArticleTitleTaxonomic revision of water-bloom-forming species of oscillatorioid cyanobacteria. Int J Syst Evol Microbiol 52 1577–1595 Occurrence Handle1:CAS:528:DC%2BD38XnvFyiurY%3D Occurrence Handle12361260

    CAS  PubMed  Google Scholar 

  38. DL Swofford (2000) PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4.0b8 Sinauer Associates Sunderland

    Google Scholar 

  39. S Turner (1997) ArticleTitleMolecular systematics of oxygenic photosynthetic bacteria. Plant Syst Evol (Supplement) 11 13–52 Occurrence Handle1:CAS:528:DyaK1cXkvVOktrc%3D

    CAS  Google Scholar 

  40. A Wilmotte M Herdman (2001) Phylogenetic relationships among the Cyanobacteria based on 16S rRNA sequences. DR Boone RW Castenholz (Eds) Bergey’s Manual of Systematic Bacteriology, vol. 1 Springer- Verlag New York 487–493

    Google Scholar 

  41. E Vardaka M Moustaka-Gouni T Lanaras (2000) ArticleTitleTemporal and spatial distribution of planktic cyanobacteria in Lake Kastoria, Greece, a shallow, urban lake. Nord J Bot 20 501–511

    Google Scholar 

Download references

Acknowledgments

This study was supported by the EU programs CYANOTOX (IC18-CT98-0293) and MIDI-CHIP (EKV2-CT-1999-00026), the Academy of Finland to KS and the Bodossaki Foundation, Greece, to SG. We thank Dr David Fewer for his help in constructing the phylogenetic trees and Dr Christina Lyra for her constructive comments about the manuscript. We thank Electron Microscopy Unit of the Institute of Biotechnology-University of Helsinki for providing laboratory facilities and Dr. Eija Jokitalo and Merja Lindman for help in EM work. Prof. Jiri Komárek is gratefully acknowledged for valuable and fruitful discussions.

Author information

Author notes

  1. E. Vardaka

    Present address: Department of Fisheries and Aquaculture Technology, Technological Educational Institute of Thessaloniki, Campus of Moudania, P.O. Box 157, GR-632 00 N, Moudania, Greece

Authors and Affiliations

  1. Department of Applied Chemistry and Microbiology, Viikki Biocenter, FIN-00014 Helsinki University, P.O. Box 56, Finland

    S. Gkelis, P. Rajaniemi & K. Sivonen

  2. Department of Botany, University of Thessaloniki, P.O. Box 109, GR-541 24, Thessaloniki, Greece

    S. Gkelis, E. Vardaka, M. Moustaka-Gouni & T. Lanaras

Authors
  1. S. Gkelis
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Rajaniemi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Vardaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Moustaka-Gouni
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. T. Lanaras
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. K. Sivonen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to K. Sivonen.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gkelis, S., Rajaniemi, P., Vardaka, E. et al. Limnothrix redekei (Van Goor) Meffert (Cyanobacteria) Strains from Lake Kastoria, Greece Form a Separate Phylogenetic Group. Microb Ecol 49, 176–182 (2005). https://doi.org/10.1007/s00248-003-2030-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00248-003-2030-7

Keywords

Search

Navigation