Abstract
Microbial hyaluronic acid (HA), commonly produced by pathogenic Streptococcus, was made possible to be produced by a generally recognized as safe Lactococcus lactis by coexpressing HA synthase and uridine diphosphate–glucose dehydrogenase (UDP-GlcDH) of Streptococcus equi subsp. zooepidemicus in a nisin-controlled expression (NICE) system. With scarce expressed HA synthase alone, the constructed recombinant L. lactis (LL-NA) strain could produce HA with a concentration about 0.08 g/l in the M17 medium supplemented with 1% (w/v) glucose. In contrast to HA synthase, UDP-GlcDH of Streptococcus could be overexpressed in the NICE system. With coexpression of heterologous UDP-GlcDH with HA synthase, the constructed LL-NAB strain grew slightly slower to a concentration about 10% lower that of the LL-NA strain. However, the HA concentration produced was enhanced about eightfold to 0.65 g/l.
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The authors thank the National Science Consul of Taiwan for financial support (NSC 95-2221-E-011-119).
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Chien, LJ., Lee, CK. Hyaluronic acid production by recombinant Lactococcus lactis . Appl Microbiol Biotechnol 77, 339–346 (2007). https://doi.org/10.1007/s00253-007-1153-z
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DOI: https://doi.org/10.1007/s00253-007-1153-z