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L-Arabinose isomerase and its use for biotechnological production of rare sugars

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Abstract

L-Arabinose isomerase (AI), a key enzyme in the microbial pentose phosphate pathway, has been regarded as an important biological catalyst in rare sugar production. This enzyme could isomerize L-arabinose into L-ribulose, as well as D-galactose into D-tagatose. Both the two monosaccharides show excellent commercial values in food and pharmaceutical industries. With the identification of novel AI family members, some of them have exhibited remarkable potential in industrial applications. The biological production processes for D-tagatose and L-ribose (or L-ribulose) using AI have been developed and improved in recent years. Meanwhile, protein engineering techniques involving rational design has effectively enhanced the catalytic properties of various AIs. Moreover, the crystal structure of AI has been disclosed, which sheds light on the understanding of AI structure and catalytic mechanism at molecular levels. This article reports recent developments in (i) novel AI screening, (ii) AI-mediated rare sugar production processes, (iii) molecular modification of AI, and (iv) structural biology study of AI. Based on previous reports, an analysis of the future development has also been initiated.

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References

  • Ahmed Z, Shimonishi T, Bhuiyan SH, Utamura M, Takada G, Izumori K (1999) Biochemical preparation of L-ribose and L-arabinose from ribitol: a new approach. J Biosci Bioeng 88(4):444–448

    Article  PubMed  CAS  Google Scholar 

  • Beadle JR, Saunders JP, Wajda TJ (1991) Process for manufacturing tagatose. US Patent 5:002,612

    Google Scholar 

  • Beerens K, Desmet T, Soetaert W (2012) Enzymes for the biocatalytic production of rare sugars. J Ind Microbiol Biotechnol 39(6):823–834

    Article  PubMed  CAS  Google Scholar 

  • Buemann B, Toubro S, Holst JJ, Rehfeld JF, Bibby BM, Astrup A (2000) D-Tagatose, a stereoisomer of D-fructose, increases blood uric acid concentration. Metabolism 49(8):969–976

    Article  PubMed  CAS  Google Scholar 

  • Burdock GA, Carabin IG (2004) Generally recognized as safe (GRAS): history and description. Toxicol Lett 150(1):3–18

    Article  PubMed  CAS  Google Scholar 

  • Cheetham PSJ, Wootton AN (1993) Bioconversion of D-galactose into D-tagatose. Enzyme Microb Technol 15:105–108

    Article  CAS  Google Scholar 

  • Cheng L, Mu W, Zhang T, Jiang B (2010a) An L-arabinose isomerase from Acidothermus cellulolytics ATCC 43068: cloning, expression, purification, and characterization. Appl Microbiol Biotechnol 86(4):1089–1097

    Article  PubMed  CAS  Google Scholar 

  • Cheng L, Mu W, Jiang B (2010b) Thermostable L-arabinose isomerase from Bacillus stearothermophilus IAM11001 for D-tagatose production: gene cloning, purification and characterisation. J Sci Food Agric 90(8):1327–1333

    Article  PubMed  CAS  Google Scholar 

  • Cheng H, Wang H, Lv J, Jiang M, Lin S, Deng Z (2011) A novel method to prepare L-arabinose from xylose mother liquor by yeast-mediated biopurification. Microb cell fact 10(1):43

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Chouayekh H, Bejar W, Rhimi M, Jelleli K, Mseddi M, Bejar S (2007) Characterization of an L-arabinose isomerase from the Lactobacillus plantarum NC8 strain showing pronounced stability at acidic pH. FEMS Microbiol Lett 277:260–267

    Article  PubMed  CAS  Google Scholar 

  • Chouayekh H, Serror P, Boudebbouze S, Maguin E (2009) Highly efficient production of the staphylococcal nuclease reporter in Lactobacillus bulgaricus governed by the promoter of the hlbA gene. FEMS Microbiol Lett 293(2):232–239

    Article  PubMed  CAS  Google Scholar 

  • De Muynck C, Beauprez J, Soetaert W, Vandamme EJ (2006) Boric acid as a mobile phase additive for high performance liquid chromatography separation of ribose, arabinose and ribulose. J Chromatogr A 1101(1):115–121

    Article  PubMed  Google Scholar 

  • Donner TW, Wilber JF, Ostrowski D (1999) D-Tagatose, a novel hexose: acute effects on carbohydrate tolerance in subjects with and without type 2 diabetes. Diabetes Obes Metab 1:285–291

    Article  PubMed  CAS  Google Scholar 

  • Fan C, Liu K, Zhang T, Zhou L, Xue D, Jia B, Mu W (2014) Biochemical characterization of a thermostable L-arabinose isomerase from a thermoacidophilic bacterium Alicyclobacillus hesperidum URH17-3-68. J Mol Catal B Enzym 102:120–126

    Article  CAS  Google Scholar 

  • Granström TB, Takata G, Tokuda M, Izumori K (2004) Izumoring: a novel and complete strategy for bioproduction of rare sugars. J Biosci Bioeng 97(2):89–94

    Article  PubMed  Google Scholar 

  • Heath EC, Horecker BL, Smyrniotis PZ, Takagi Y (1958) Pentose fermentation by Lactobacillus plantarum II. L-arabinose isomerase. J Biol Chem 231(2):1031–1037

    PubMed  CAS  Google Scholar 

  • Helanto M, Kiviharju K, Leisola M, Nyyssölä A (2007) Metabolic engineering of Lactobacillus plantarum for production of L-ribulose. Appl Environ Microbiol 73(21):7083–7091

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Helanto M, Kiviharju K, Granström T, Leisola M, Nyyssola A (2009) Biotechnological production of L-ribose from L-arabinose. Appl Microb Biotechnol 83(1):77–83

    Article  CAS  Google Scholar 

  • Hong YH, Lee DW, Lee SJ, Choe EA, Kim SB, Lee YH, Cheigh CI, Pyun YR (2007) Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana. Biotechnol Lett 29(4):569–574

    Article  PubMed  CAS  Google Scholar 

  • Hong YH, Lee DW, Pyun YR, Lee SH (2011) Creation of metal-independent hyper thermophilic L-arabinose isomerase by homologous recombination. J Agric Food Chem 59(24):12939–12947

    Article  PubMed  CAS  Google Scholar 

  • Hu C, Li L, Zheng Y, Rui L, Hu C (2011) Perspectives of biotechnological production of L-ribose and its purification. Appl Microbiol Biotechnol 92:449–455

    Article  PubMed  CAS  Google Scholar 

  • Hung XG, Tseng WC, Liu SM, Tzou WS, Fang TY (2014) Characterization of a thermophilic L-arabinose isomerase from Thermoanaerobacterium saccharolyticum NTOU1. Biochem Eng J 83:121–128

    Article  CAS  Google Scholar 

  • Ibrahim OO, Spradlin JE (2000) Process for manufacturing D-tagatose. US Patent 6:057,135

    Google Scholar 

  • Izumori K, Tsuzaki K (1988) Production of D-tagatose from D-galactitol by Mycobacterium smegmatis. J Ferment Technol 66(2):225–227

    Article  CAS  Google Scholar 

  • Jebors S, Tauran Y, Aghajari N, Boudebbouze S, Maguin E, Haser R, Coleman AW, Rhimi M (2011) Supramolecular stabilization of acid tolerant L-arabinose isomerase from Lactobacillus sakei. Chem Commun 47(45):12307–12309

    Article  CAS  Google Scholar 

  • JØrgensen F, Hansen OC, Stougaard P (2004) Enzymatic conversion of D-galactose to D-tagatose: heterologous expression and characterisation of a thermostable L-arabinose isomerase from Thermoanaerobacter mathranii. Appl Microbiol Biotechnol 64(6):816–822

    Article  PubMed  Google Scholar 

  • Jung ES, Kim HJ, Oh DK (2005) Tagatose production by immobilized recombinant Escherichia coli cells containing Geobacillus stearothermophilus L-arabinose isomerase mutant in a packed-bed bioreactor. Biotechnol Prog 21(4):1335–1340

    Article  PubMed  CAS  Google Scholar 

  • Kagawa KI, Okayama KT (2001) L-Ribose isomerase, its preparation and uses. US patent 6,294,369 B1

  • Kim P (2004) Current studies on biological tagatose production using L-arabinose isomerase: a review and future perspective. Appl Microb Biotechnol 65(3):243–249

    CAS  Google Scholar 

  • Kim HJ, Oh DK (2005) Purification and characterization of an L-arabinose isomerase from an isolated strain of Geobacillus thermodenitrificans producing D-tagatose. J Biotechnol 120(2):162–173

    Article  PubMed  CAS  Google Scholar 

  • Kim P, Yoon SH, Roh HJ, Choi JH (2001a) High production of D-tagatose, a potential sugar substitute, using immobilized L-arabinose isomerase. Biotechnol Prog 17(1):208–210

    Article  PubMed  CAS  Google Scholar 

  • Kim P, Yoon S, Seo M, Oh DK, Choi JH (2001b) Improvement of tagatose conversion rate by genetic evolution of thermostable galactose isomerase. Biotechnol Appl Biochem 34:99–102

    Article  PubMed  CAS  Google Scholar 

  • Kim BC, Lee YH, Lee HS, Lee DW, Choe EA, Pyun YR (2002) Cloning, expression and characterization of L-arabinose isomerase from Thermotoga neapolitana: bioconversion of D-galactose to D-tagatose using the enzyme. FEMS Microbiol Lett 212(1):121–126

    PubMed  CAS  Google Scholar 

  • Kim JW, Kim YW, Roh HJ, Kim HY, Cha JH, Park KH, Park CS (2003a) Production of tagatose by a recombinant thermostable L-arabinose isomerase from Thermus sp. IM6501. Biotechnol Lett 25(12):963–967

    Article  PubMed  CAS  Google Scholar 

  • Kim HJ, Ryu SA, Kim P, Oh DK (2003b) A feasible enzymatic process for D-tagatose production by an immobilized thermostable L-arabinose isomerase in a packed-bed bioreactor. Biotechnol Prog 19(2):400–404

    Article  PubMed  CAS  Google Scholar 

  • Kim HJ, Kim JH, Oh HJ, Oh DK (2006) Characterization of a mutated Geobacillus stearothermophilus L-arabinose isomerase that increases the production rate of D-tagatose. J Appl Microbiol 101(1):213–221

    Article  PubMed  CAS  Google Scholar 

  • Kim SB, Lee YM, Park SW, Kim JH, Song SH, Lee KP (2009) Recombinant GRAS strains expressing thermophilic arabinose isomerase as an active form and method of preparing food grade tagatose by using the same. US Patent 8:137–946, B2

    Google Scholar 

  • Kim JH, Prabhu P, Jeya M, Tiwari MK, Moon HJ, Singh RK, Lee JK (2010) Characterization of an L-arabinose isomerase from Bacillus subtilis. Appl Microbiol Biotechnol 85(6):1839–1847

    Article  PubMed  CAS  Google Scholar 

  • Kim BJ, Hong SH, Shin KC, Jo YS, Oh DK (2014a) Characterization of a F280N variant of L-arabinose isomerase from Geobacillus thermodenitrificans identified as a D-galactose isomerase. Appl Microbiol Biotechnol 1–11

  • Kim KR, Seo ES, Oh DK (2014b) L-Ribose production from L-arabinose by immobilized recombinant Escherichia coli co-expressing the L-arabinose isomerase and mannose-6-phosphate isomerase genes from Geobacillus thermodenitrificans. Appl Biochem Biotechnol 172(1):275–288

    Article  PubMed  CAS  Google Scholar 

  • Lee N, Gielow W, Martin R, Hamilton E, Fowler A (1986) The organization of the araBAD operon of Escherichia coli. Gene 47(2):231–244

    Article  PubMed  CAS  Google Scholar 

  • Lee DW, Jang HJ, Choe EA, Kim BC, Lee SJ, Kim SB, Hong YH, Pyun YR (2004) Characterization of a thermostable L-arabinose (D-galactose) isomerase from the hyper thermophilic eubacterium Thermotoga maritima. Appl Environ Microbiol 70:1397–1404

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Lee DW, Choe EA, Kim SB, Eom SH, Hong YH, Lee SJ, Lee HS, Lee DY, Ryun YR (2005a) Distinct metal dependence for catalytic and structural functions in the L-arabinose isomerases from the mesophilic Bacillus halodurans and the thermophilic Geobacillus stearothermophilus. Arch Biochem Biophys 434(2):333–343

    Article  PubMed  CAS  Google Scholar 

  • Lee SJ, Lee DW, Choe EA, Hong YH, Kim SB, Kim BC, Pyun YR (2005b) Characterization of a thermoacidophilic L-arabinose isomerase from Alicyclobacillus acidocaldarius: role of Lys-269 in pH optimum. Appl Environ Microbiol 71:7888–7896

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Lee GY, Jung JH, Seo DH, Hansin J, Ha SJ, Cha J, Kim YS, Park CS (2011) Isomaltulose production via yeast surface display of sucrose isomerase from Enterobacter sp. FMB-1 on Saccharomyces cerevisiae. Bioresour Technol 102(19):9179–9184

    Article  PubMed  CAS  Google Scholar 

  • Lee SJ, Lee SJ, Lee YJ, Kim SB, Kim SK, Lee DW (2012) Homologous alkalophilic and acidophilic L-arabinose isomerases reveal region-specific contributions to the pH dependence of activity and stability. Appl Environ Microbiol 78(24):8813–8816

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Levin GV (2002) Tagatose, the new GRAS sweetener and health product. J Med Food 5(1):23–36

    Article  PubMed  CAS  Google Scholar 

  • Li Y, Zhu Y, Liu A, Sun Y (2011) Identification and characterization of a novel L-arabinose isomerase from Anoxybacillus flavithermus useful in D-tagatose production. Extremophiles 15:441–450

    Article  PubMed  Google Scholar 

  • Li G, Xu Z, Li S, Xu H (2012) Probing the essential catalytic residues and substrate affinity in thermophilic L-arabinose isomerase by homology modeling and site-directed mutagenesis. Chin J Catal 33:1717–1723

    CAS  Google Scholar 

  • Liang M, Chen M, Liu X, Zhai Y, Liu X, Zhang H, Xiao M, Wang P (2012) Bioconversion of D-galactose to D-tagatose: continuous packed bed reaction with an immobilized thermostable L-arabinose isomerase and efficient purification by selective microbial degradation. Appl Microbiol Biotechnol 93(4):1469–1474

    Article  PubMed  CAS  Google Scholar 

  • Lim BC, Kim HJ, Oh DK (2007) High production of D-tagatose by the addition of boric acid. Biotechnol Prog 23:824–828

    Article  PubMed  CAS  Google Scholar 

  • Lim BC, Kim HJ, Oh DK (2008) Tagatose production with pH control in a stirred tank reactor containing immobilized L-arabinose isomerase from Thermotoga neapolitana. Appl Biochem Biotechnol 149(3):245–253

    Article  PubMed  CAS  Google Scholar 

  • Lim YR, Yeom SJ, Oh DK (2012) Production of L-ribose from L-ribulose by a triple-site variant of mannose-6-phosphate isomerase from Geobacillus thermodenitrificans. Appl Environ Microbiol 78(11):3880–3884

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Liu Y, Li S, Xu H, Wu L, Xu Z, Liu J, Feng XH (2014) Efficient production of D-tagatose using a food-grade surface display system. J Agric Food Chem DOI: 10.1021/jf501937j

  • Manjasetty BA, Chance MR (2006) Crystal structure of Escherichia coli L-arabinose isomerase (ECAI), the putative target of biological tagatose production. J Mol Biol 360:297–309

    Article  PubMed  CAS  Google Scholar 

  • Men Y, Zhu Y, Zhang L, Kang Z, Izumori K, Sun Y, Ma Y (2014) Enzymatic conversion of D-galactose to D-tagatose: cloning, overexpression and characterization of L-arabinose isomerase from Pediococcus pentosaceus PC-5. Microbiol Res 169(2):171–178

    Article  PubMed  CAS  Google Scholar 

  • Oh DK (2007) Tagatose: properties, applications, and biotechnological processes. Appl Microbiol Biotechnol 76:1–8

    Article  PubMed  CAS  Google Scholar 

  • Oh HJ, Kim HJ, Oh DK (2006a) Increase in D-tagatose production rate by site-directed mutagenesis of L-arabinose isomerase from Geobacillus thermodenitrificans. Biotechnol Lett 28(3):145–149

    Article  PubMed  CAS  Google Scholar 

  • Oh DK, Oh HJ, Kim HJ, Cheon J, Kim P (2006b) Modification of optimal pH in L-arabinose isomerase from Geobacillus stearothermophilus for D-galactose isomerization. J Mol Catal B Enzym 43:108–112

    Article  CAS  Google Scholar 

  • Okano K (2009) Synthesis and pharmaceutical application of L-ribose. Tetrahedron 65(10):1937–1949

    Article  CAS  Google Scholar 

  • Prabhu P, Tiwari MK, Jeya M, Gunasekaran P, Kim IW, Lee JK (2008) Cloning and characterization of a novel L-arabinose isomerase from Bacillus licheniformis. Appl Microbiol Biotechnol 81(2):283–290

    Article  PubMed  CAS  Google Scholar 

  • Prabhu P, Jeya M, Lee JK (2010) Probing the molecular determinant for the catalytic efficiency of L-arabinose isomerase from Bacillus licheniformis. Appl Environ Microbiol 76(5):1653–1660

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Rhimi M, Bejar S (2006) Cloning, purification and biochemical characterization of metallic-ions independent and thermoactive L-arabinose isomerase from the Bacillus stearothermophilus US100 strain. Biochim Biophys Acta 1760:191–199

    Article  PubMed  CAS  Google Scholar 

  • Rhimi M, Juy M, Aghajari N, Haser R, Bejar S (2007) Probing the essential catalytic residues and substrate affinity in the thermoactive Bacillus stearothermophilus US100 L-arabinose isomerase by site-directed mutagenesis. J Bacteriol 189(9):3556–3563

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Rhimi M, Aghajari N, Juy M, Chouayekh H, Maguin E, Haser R, Bejar S (2009) Rational design of Bacillus stearothermophilus US100 L-arabinose isomerase: potential applications for D-tagatose production. Biochimie 91(5):650–653

    Article  PubMed  CAS  Google Scholar 

  • Rhimi M, lhammami R, Bajic G, Boudebbouze S, Maguin E, Haser R, Aghajari N (2010) The acid tolerant L-arabinose isomerase from the food grade Lactobacillus sakei 23K is an attractive D-tagatose producer. Bioresour Technol 101:9171–9177

    Article  PubMed  CAS  Google Scholar 

  • Rhimi M, Bajic G, Ilhammami R, Boudebbouze S, Maguin E, Haser R, Aghajari N (2011a) The acid-tolerant L-arabinose isomerase from the mesophilic Shewanella sp. ANA-3 is highly active at low temperatures. Microb Cell Fact 10:96

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Rhimi M, Chouayekh H, Gouillouard I, Maguin E, Bejar S (2011b) Production of D-tagatose, a low caloric sweetener during milk fermentation using L-arabinose isomerase. Bioresour Technol 102:3309–3315

    Article  PubMed  CAS  Google Scholar 

  • Rollini M, Manzoni M (2005) Bioconversion of D-galactitol to tagatose and dehydrogenase activity induction in Gluconobacter oxydans. Process Biochem 40(1):437–444

    Article  CAS  Google Scholar 

  • Ryu SA, Kim CS, Kim HJ, Baek DH, Oh DK (2003) Continuous D-tagatose production by immobilized thermostable L-arabinose isomerase in a packed-bed bioreactor. Biotechnol Prog 19:1643–1647

    Article  PubMed  CAS  Google Scholar 

  • Salonen N, Nyyssölä A, Salonen K, Turunen O (2012) Bifidobacterium longum L-arabinose isomerase—overexpression in Lactococcus lactis, purification, and characterization. Appl Biochem Biotechnol 168(2):392–405

    Article  PubMed  CAS  Google Scholar 

  • Salonen N, Salonen K, Leisola M, Nyyssölä A (2013) D-Tagatose production in the presence of borate by resting Lactococcus lactis cells harboring Bifidobacterium longum L-arabinose isomerase. Bioproc Biosyst Eng 1–9

  • Sirén N, Salonen K, Leisola M, Nyyssola A (2008) A new and efficient phosphate starvation inducible expression system for Lactococcus lactis. Appl Microbiol Biotechnol 79(5):803–810

    Article  PubMed  Google Scholar 

  • Staudigl P, Haltrich D, Peterbauer CK (2014) L-Arabinose isomerase and D-xylose isomerase from Lactobacillus reuteri: characterization, coexpression in the food grade host Lactobacillus plantarum, and application in the conversion of D-galactose and D-glucose. J Agric Food Chem 62(7):1617–1624

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Wallace LJ, Eiserling FA, Wilcox G (1978) The shape of L-arabinose isomerase from Escherichia coli. J Biol Chem 253(10):3717–3720

    PubMed  CAS  Google Scholar 

  • Wanarska M, Kur J (2012) A method for the production of D-tagatose using a recombinant Pichia pastoris strain secreting β-D-galactosidase from Arthrobacter chlorophenolicus and a recombinant L-arabinose isomerase from Arthrobacter sp. 22c. Microb Cell Fact 11(1):113

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Xu Z, Qing YJ, Li S, Feng XH, Xu H, Ouyang PK (2011) A novel L-arabinose isomerase from Lactobacillus fermentum CGMCC2921 for D-tagatose production: gene cloning, purification and characterization. J Mol Catal B Enzym 70:1–7

    Article  CAS  Google Scholar 

  • Xu Z, Li S, Fu FG, Li GX, Feng X, Xu H, Ouyang PK (2012) Production of D-tagatose, a functional sweetener, utilizing alginate immobilized Lactobacillus fermentum CGMCC2921 cells. Appl Biochem Biotechnol 166:961–973

    Article  PubMed  CAS  Google Scholar 

  • Xu Z, Li S, Feng X, Zhan Y, Xu H (2014) Function of aspartic acid residues in optimum pH control of L-arabinose isomerase from Lactobacillus fermentum. Appl Microbiol Biotechnol 1–10

  • Yeom SJ, Ji JH, Kim NH, Park CS, Oh DK (2009a) Substrate specificity of a mannose-6-phosphate isomerase from Bacillus subtilis and its application in the production of L-ribose. Appl Environ Microbiol 75(14):4705–4710

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Yeom SJ, Kim NH, Park CS, Oh DK (2009b) L-Ribose production from L-arabinose by using purified L-arabinose isomerase and mannose-6-phosphate isomerase from Geobacillus thermodenitrificans. Appl Environ Microbiol 75(21):6941–6943

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Yeom SJ, Seo ES, Kim BN, Kim YS, Oh DK (2011) Characterization of a mannose-6-phosphate isomerase from Thermus thermophilus and increased L-ribose production by its R142N mutant. Appl Environ Microbiol 77(3):762–767

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Yoon SH, Kim P, Oh DK (2003) Properties of L-arabinose isomerase from Escherichia coli as biocatalyst for tagatose production. World J Microbiol Biotechnol 19(1):47–51

    Article  CAS  Google Scholar 

  • Zehner LR (1988) D-tagatose as a low-calorie carbohydrate sweetener and bulking agent. US Patent 4:786,722

    Google Scholar 

  • Zhan Y, Xu Z, Li S, Liu X, Xu L, Feng X, Xu H (2014) Coexpression of β-D-galactosidase and L-arabinose isomerase in the production of D-tagatose: a functional sweetener. J Agric Food Chem 62(11):2412–2417

    Article  PubMed  CAS  Google Scholar 

  • Zhang YW, Prabhu P, Lee JK (2010a) Alginate immobilization of recombinant Escherichia coli whole cells harboring L-arabinose isomerase for L-ribulose production. Bioproc Biosyst Eng 33(6):741–748

    Article  CAS  Google Scholar 

  • Zhang YW, Jeya M, Lee JK (2010b) L-Ribulose production by an Escherichia coli harboring L-arabinose isomerase from Bacillus licheniformis. Appl Microbiol Biotechnol 87(6):1993–1999

    Article  PubMed  CAS  Google Scholar 

  • Zhang YW, Jeya M, Lee JK (2011) Enhanced activity and stability of L-arabinose isomerase by immobilization on aminopropyl glass. Appl Microbiol Biotechnol 89(5):1435–1442

    Article  PubMed  CAS  Google Scholar 

  • Zhou X, Wu JC (2012) Heterologous expression and characterization of Bacillus coagulans L-arabinose isomerase. World J Microbiol Biotechnol 28(5):2205–2212

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This work was supported by the National High Technology Research and Development Program of China (2012AA021503), the National Natural Science Foundation of China (31371732 and C010202), the Natural Science Foundation of Jiangsu Province (BK20140933), the China Postdoctoral Science Foundation (2014M551582), and the State Key Laboratory of Materials-Oriented Chemical Engineering (KL13-06).

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  1. State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing, 210009, People’s Republic of China

    Zheng Xu, Sha Li, Xiaohai Feng, Jinfeng Liang & Hong Xu

  2. College of Food Science and Light Industry, Nanjing Tech University, Nanjing, 211816, People’s Republic of China

    Zheng Xu, Sha Li, Xiaohai Feng, Jinfeng Liang & Hong Xu

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Correspondence to Hong Xu.

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Xu, Z., Li, S., Feng, X. et al. L-Arabinose isomerase and its use for biotechnological production of rare sugars. Appl Microbiol Biotechnol 98, 8869–8878 (2014). https://doi.org/10.1007/s00253-014-6073-0

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