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Characterization of Phosphate-Solubilizing Fluorescent Pseudomonads from the Rhizosphere of Seabuckthorn Growing in the Cold Deserts of Himalayas

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Abstract

Isolation and characterization of fluorescent pseudomonads with high phosphate-solubilizing ability is reported from the alkaline and calcium-rich soils with low P availability in the cold desert region of Lahaul and Spiti in the trans-Himalayas of India. Of 216 phosphate-solubilizing isolates, 12 exhibiting high solubilization of tricalcium phosphate (TCP) in NBRIP liquid culture were identified as Pseudomonas trivialis, P. poae, P. fluorescens, and Pseudomonas spp. on the basis of phenotypic features, whole-cell fatty acids methyl ester (FAME) profiles, and 16S rDNA sequencing. These isolates also showed relatively high solubilization of North Carolina rock phosphate (NCRP) in comparison to the solubilization of Mussoorie rock phosphate (MRP) and Udaipur rock phosphate (URP). The solubilization of phosphate substrates by P. trivialis and P. poae is reported for the first time.

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Acknowledgments

The authors acknowledge the Microbial Type Culture Collection and Gene Bank, Institute of Microbial Technology, Chandigarh, India, for FAME analysis. They also acknowledge the Director of the Institute of Himalayan Bioresource Technology for providing the necessary facilities, and the Department of Biotechnology, Government of India for the financial support. Thanks are also due to Mr. Digvijaya Singh Naruka for the technical support in operating the DNA sequencer.

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  1. Institute of Himalayan Bioresource Technology, P.O. Box No. 6, Palampur, HP, 176 061, India

    Arvind Gulati, Praveen Rahi & Pratibha Vyas

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  1. Arvind Gulati
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  2. Praveen Rahi
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  3. Pratibha Vyas
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Correspondence to Arvind Gulati.

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Gulati, A., Rahi, P. & Vyas, P. Characterization of Phosphate-Solubilizing Fluorescent Pseudomonads from the Rhizosphere of Seabuckthorn Growing in the Cold Deserts of Himalayas. Curr Microbiol 56, 73–79 (2008). https://doi.org/10.1007/s00284-007-9042-3

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  • DOI: https://doi.org/10.1007/s00284-007-9042-3

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