Abstract
Chinese sacbrood virus (CSBV) is the pathogen of Chinese sacbrood disease, which poses a serious threat to honeybee Apis cerana, and tends to cause bee colony and even the whole apiary collapse. Here we report on prevention of CSBV infection by feeding second instar larvae of A. cerana with specific sequences of CSBV double-stranded RNA (dsRNA). Protection of the bee larvae from CSBV by ingestion of CSBV-derived dsRNA was further demonstrated by quantitative real-time PCR (qRT-PCR) and northern blot analysis. The result provides a potential method to protect A. cerana from CSBV infection.
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References
Schmid-Hempel P (1998) Colony performance and immunocompetence of a social insect, Bombus terrestris, in poor and variable environments. Funct Ecol 12:22–30
The Honeybee Genome Sequencing Consortium (2006) Insights into social insects from the genome of the honeybee A. mellifera. Nature 443:941–949
Berényi O, Bakonyi T, Derakhshifar I, Köglberger H, Nowotny N (2006) Occurrence of six honeybee viruses in diseased Austrian apiaries. Appl Environ Microbiol 72:2414–2420
Chen YP, Pettis JS, Collins A, Feldlaufer MF (2006) Prevalence and transmission of honeybee viruses. Appl Environ Microbiol 72:606–611
Shen MQ, Cui LW, Ostiguy N, Cox-Foster D (2005) Intricate transmission routes and interactions between picorna-like viruses (Kashmir bee virus and sacbrood virus) with the honeybee host and the parasitic Varroa mite. J Gen Virol 86:2281–2289
Allen MF, Ball BV (1996) The incidence and world distribution of the honey bee viruses. Bee World 77:141–162
Bailey L, Ball BV (1991) Honey bee pathology. Academic Press, London, UK
Dong BY, Fang YZ, Guo ZQ, Li XJ, Zhang Y, Bi KH (1986) Study on Chinese sacbrood bee virus. Apic China 2:6–8
Ongus JR, Peters D, Bonmatin JM, Bengsch E, Vlak JM, Oers MM (2004) Complete sequence of a picorna-like virus of the genus Iflavirus replicating in the mite Varroa destructor. J Gen Virol 85:3747–3755
Yang GH, Zhang GZ, Du ZL (1979) The pathogen identification of the Chinese Sacbrood bee larvae disease. Apic China 5:15–17
Bailey L (1975) Recent research on honey bee viruses. Bee World 56:55–64
Yan X, Han RC (2008) Diagnostic technologies of common pathogens of honeybees in China. Chin Bull Entomol 45:483–488
Anderson DL, Gibbs AJ (1989) Transpuparial transmission of Kashmir bee virus and sacbrood virus in the honey bee (Apis mellifera). Ann Appl Biol 114:1–7
Bailey L (1969) The multiplication and spread of sacbrood virus of bees. Ann Appl Biol 63:483–491
Zhang JQ, Feng JX, Liang YY, Chen DH, Zhou ZH, Zhang QF (2001) Three-dimensional structure of the Chinese sacbrood bee virus. Sci China 31:367–371
Dong BY, Yuan YD, Zhang GC, Guo ZQ, Liu JS (1984) Study on serological relationships or mutual susceptibility of CSBV and SBV. Apic China 3:8–9
Feng JX, Yu JX, Zhang JQ (1999) Cloning of partial structure protein gene from Chinese sacbrood virus. Acta Sci Nat Univ Sunyatseni 38:124–126
Liu NN (2009) Occurrence and control of the Chinese sacbrood bee virus. Apic China 60:26–27
Geley S, Muller C (2004) RNAi: ancient mechanism with a promising future. Exp Gerontol 39:985–998
Hannon GJ (2002) RNA interference. Nature 418:244–251
Amdam GV, Simões ZLP, Guidugli RK, Norberg K, Omholt SW (2003) Disruption of vitellogenin gene function in adult honeybees by intra-abdominal injection of double-stranded RNA. BMC Biotechnol 3:1
Schlüns H, Crozier RH (2007) Relish regulates expression of antimicrobial peptide genes in the honeybee, A. mellifera, shown by RNA interference. Insect Mol Biol 16:753–759
Beye M, Hartel S, Hagen A, Hasselmann M, Omholt SW (2002) Specific developmental gene silencing in the honey bee using a homeobox motif. Insect Mol Biol 11:527–532
Maori E, Paldi N, Shafir S, Kalev H, Tsur E, Glick E, Sela I (2009) A bee-affecting virus associated with colony collapse disorder can be silenced by dsRNA ingestion. Insect Mol Biol 18:55–60
Nunes FMF, Simões ZLP (2009) A non-invasive method for silencing gene transcription in honeybees maintained under natural conditions. Insect Biochem Mol Biol 39:157–160
Chen YP, Zhao Y, Hammond J, Hsu H, Evans JD, Feldlaufer MF (2004) Multiple virus infections in the honey bee and genome divergence of honey bee viruses. J Invertebr Pathol 87:84–93
Yan X, Chen JH, Han RC (2009) Detection of Chinese sacbrood virus (CSBV) in A. cerana by RT-PCR method. Sociobiology 53:687–694
Hong KM, Najjar H, Hawley M, Press RD (2004) Quantitative real-time PCR with automated sample preparation for diagnosis and monitoring of cytomegalovirus infection in bone barrow transplant patients. Clin Chem 50:846–856
Yao Q, Gao L, Chen KP, Hu ZG (2005) Detection of proliferation of Bombyx mori nucleopolyhedrivirus in its host by fluorescence quantitative PCR. Acta Entomol Sin 48:871–875
Patel A, Fondrk MK, Kaftanoglu O, Emore C, Hunt G, Frederick K, Amdam GV (2007) The making of a queen: TOR pathway is a key player in diphenic caste development. PLoS ONE 2:e509
Araujo RN, Santos A, Pinto FS, Gontijo NF, Lehane MJ, Pereira MH (2006) RNA interference of the salivary gland nitrophorin 2 in the triatomine bug Rhodnius prolixus (Hemiptera: Reduviidae) by dsRNA ingestion or injection. Insect Biochem Mol Biol 36:683–693
Turner CT, Davy MW, MacDiarmid RM, Plummer KM, Birch NP, Newcomb RD (2006) RNA interference in the light brown apple moth, Epiphyas postvittana (Walker) induced by double-stranded RNA feeding. Insect Mol Biol 15:383–391
Aronstein K, Pankiw T, Saldivar E (2006) SID-I is implicated in systemic gene silencing in the honey bee. J Apic Res 45:20–24
Zhang QF, Yang YF, Liang YY, Lu XY, Zhang JQ (2002) Study on structure of the Chinese Sacbrood Virus’ nucleic acid. J Chin Electron Microsc Soc 21:331–334
Koonin EV, Dolja VV (1993) Evolution and taxonomy of positive-strand RNA viruses implications of comparative analysis of amino acid sequences. Crit Rev Biochem Mol Biol 28:375–430
Geiss BJ, Pierson TC, Diamond MS (2005) Actively replicating West Nile virus is resistant to cytoplasmic delivery of siRNA. Virol J 2:53
Acknowledgments
This work was supported by National Natural Science Foundation of China (No: 30970413 and 30870346), Science and Technology Planning Project Foundation of Guangdong, China (No: 2007B020500002-4 and 2008A020100023), Science and Technology Planning Project Foundation of Guangzhou Agricultural Bureau (No: GZCQC0702FG06054), and Foundation of Guangdong Academy of Sciences (No: SF200810).
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Liu, X., Zhang, Y., Yan, X. et al. Prevention of Chinese Sacbrood Virus Infection in Apis cerana using RNA Interference. Curr Microbiol 61, 422–428 (2010). https://doi.org/10.1007/s00284-010-9633-2
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DOI: https://doi.org/10.1007/s00284-010-9633-2