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Cloning and characterization of a calcium dependent protein kinase gene associated with cotton fiber development

  • Physiology and Biochemistry
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Abstract

The gene GhCPK1 encoding a calcium dependent protein kinase was identified from cotton. Transcripts of GhCPK1 accumulated primarily in the elongating fiber, and Arabidopsis plants transformed with GhCPK1 promoter-GUS construct exhibited GUS activity mainly in the developing trichomes, roots, young leaves and sepals. In the bombarded onion epidermal cells, GhCPK1-GFP fusion proteins showed a subcellular distribution in the plasma membrane. In vitro assays indicated that GhCPK1 was a functional calcium-dependent kinase able to undergo autophosphorylation and phosphorylation of the known substrate histone III-S. Together, these results suggest that GhCPK1 may play a role in the calcium signaling events associated with fiber elongation.

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Abbreviations

CaM:

Calmodulin

CBL:

Calcineurin B-like protein

CDPK:

Calcium dependent protein kinase

DPA:

Days post anthesis

GFP:

Green fluorescent protein

GhCPK1:

Gossypium hirsutum calcium dependent protein kinase 1

GUS:

β-Glucuronidase

MS:

Murashige and Skoog medium

ORF:

Open reading frame

RACE:

Rapid amplification of cDNA end

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Acknowledgments

This work is supported by grants from the National Key Basic Research Program of China (Grant No: 2004CB117304) and the ‘‘863’’ High Technology Development Program (Grant No: 2006AA10A109-3).

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Authors and Affiliations

  1. State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, 100094, China

    Quan-Sheng Huang & Guo-Ying Wang

  2. National Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China

    Quan-Sheng Huang, Hai-Yun Wang, Peng Gao & Gui-Xian Xia

  3. National Center for Plant Gene Research, Beijing, 100101, China

    Hai-Yun Wang, Peng Gao & Gui-Xian Xia

  4. Xinjiang Academy of Agricultural Sciences, Urumuqi, Xinjiang, 830094, China

    Quan-Sheng Huang

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  1. Quan-Sheng Huang
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  2. Hai-Yun Wang
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  3. Peng Gao
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  4. Guo-Ying Wang
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  5. Gui-Xian Xia
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Correspondence to Gui-Xian Xia.

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Communicated by W.-H. Wu.

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Huang, QS., Wang, HY., Gao, P. et al. Cloning and characterization of a calcium dependent protein kinase gene associated with cotton fiber development. Plant Cell Rep 27, 1869–1875 (2008). https://doi.org/10.1007/s00299-008-0603-0

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  • DOI: https://doi.org/10.1007/s00299-008-0603-0

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