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Both PKMζ and KIBRA are closely related to reference memory but not working memory in a T-maze task in rats

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Abstract

Protein kinase M zeta (PKMζ) and the kidney and brain protein (KIBRA) play important roles in various forms of memories. However, whether they are involved in performing the T-maze task is still unknown. In this study, the delayed nonmatch-to-sample (DNMS) task in a T-maze was given to rats. The percentage of correct choices denoting the performance accuracy was calculated and the protein levels of PKMζ and KIBRA in rat’s prefrontal cortex were measured. The results showed significantly increased performance accuracy after the training phase, which was maintained on the next day in groups with a delay of 10 s but not 30 s, indicating that 30 s is too long for rats to maintain working memory. As for the expressions of PKMζ and KIBRA, significant increases were observed 1 day after the training phase, indicating that the formation of reference memory accompanies an increase in PKMζ and KIBRA. No significant difference was found among groups with various delay intervals, indicating that the expressions of PKMζ and KIBRA exert no effects on the performance of working memory. These results provide the first evidence that KIBRA as well as PKMζ is closely related to reference memory but not working memory in rats.

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Acknowledgments

This work was supported by a grant from the Kaohsiung Medical University Research Foundation (KMU-M110003), Taiwan. All experimental procedures have been reviewed and approved by the Animal Care and Use Committee of the Kaohsiung Medical University.

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Authors and Affiliations

  1. Department of Sports Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, 807, Taiwan

    Dean-Chuan Wang

  2. Department of Physiology, College of Medicine, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, Kaohsiung, 807, Taiwan

    Pei-Chun Liu, Hui-Shan Hung & Tsan-Ju Chen

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  1. Dean-Chuan Wang
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  2. Pei-Chun Liu
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  3. Hui-Shan Hung
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  4. Tsan-Ju Chen
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Correspondence to Tsan-Ju Chen.

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Wang, DC., Liu, PC., Hung, HS. et al. Both PKMζ and KIBRA are closely related to reference memory but not working memory in a T-maze task in rats. J Comp Physiol A 200, 77–82 (2014). https://doi.org/10.1007/s00359-013-0862-2

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  • DOI: https://doi.org/10.1007/s00359-013-0862-2

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