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Sequence homogenization and chromosomal localization of VicTR-B satellites differ between closely related Vicia species

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Abstract

Satellite sequences of the VicTR-B family are specific for the genus Vicia (Leguminosae), but their abundance varies among the species, being the highest in Vicia sativa and Vicia grandiflora. In this study, we have sequenced multiple randomly cloned VicTR-B fragments from these two species and analyzed their sequence variability, periodicity, and chromosomal localization. We have found that V. sativa VicTR-B sequences are homogeneous with respect to their nucleotide sequences and periodicity (monomers of 38 bp), whereas V. grandiflora repeats are considerably more variable, occurring in at least four distinct sequence subfamilies. Although the periodicity of 38 bp was conserved in most of the V. grandiflora sequences, one of the subfamilies was composed of higher-order repeats of 186 bp, which originated from a pentamer of the basic repeated unit. Individual VicTR-B subfamilies were preferentially located in either intercalary or subtelomeric regions of chromosomes. Interestingly, two V. grandiflora subfamilies with the highest similarity to V. sativa VicTR-B sequences were located in intercalary heterochromatic bands, showing similar chromosomal distribution as the majority of VicTR-B repeats in V. sativa. The other two V. grandiflora subfamilies showing a considerable divergence from V. sativa sequences were found to be accumulated at subtelomeric regions of V. grandiflora chromosomes.

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Acknowledgements

We thank Ms. J. Látalová and Ms. H. Štěpančíková for excellent technical assistance. This work was supported by grants GA204/04/1207 from the Czech Science Foundation and AVOZ50510513 from the Academy of Sciences of the Czech Republic. The experiments were carried out in compliance with the current laws governing genetic experimentation in the Czech Republic.

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  1. Institute of Plant Molecular Biology, Branišovská 31, České Budějovice, 37005, Czech Republic

    Jiří Macas, Alice Navrátilová & Andrea Koblížková

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  1. Jiří Macas
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  2. Alice Navrátilová
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  3. Andrea Koblížková
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Correspondence to Jiří Macas.

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Communicated by I. Schubert

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Macas, J., Navrátilová, A. & Koblížková, A. Sequence homogenization and chromosomal localization of VicTR-B satellites differ between closely related Vicia species. Chromosoma 115, 437–447 (2006). https://doi.org/10.1007/s00412-006-0070-8

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  • DOI: https://doi.org/10.1007/s00412-006-0070-8

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