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Transformation-associated recombination (TAR) cloning for genomics studies and synthetic biology

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Abstract

Transformation-associated recombination (TAR) cloning represents a unique tool for isolation and manipulation of large DNA molecules. The technique exploits a high level of homologous recombination in the yeast Sacharomyces cerevisiae. So far, TAR cloning is the only method available to selectively recover chromosomal segments up to 300 kb in length from complex and simple genomes. In addition, TAR cloning allows the assembly and cloning of entire microbe genomes up to several Mb as well as engineering of large metabolic pathways. In this review, we summarize applications of TAR cloning for functional/structural genomics and synthetic biology.

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Acknowledgments

We thank Dr. Nicolas Lee for helping in preparation of figures.

This work was supported by the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research, USA (V.L. and N.K).

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Authors and Affiliations

  1. Developmental Therapeutics Branch, National Cancer Institute, Bethesda, MD, 20892, USA

    Natalay Kouprina & Vladimir Larionov

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  1. Natalay Kouprina
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  2. Vladimir Larionov
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Correspondence to Natalay Kouprina.

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The authors declare that they have no conflict of interest. This article does not contain any studies with human participants or animals performed by any of the authors.

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This article is related to the 21st International Chromosome Conference (Foz do Iguaçu, Brazil, July 10–13, 2016).

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Kouprina, N., Larionov, V. Transformation-associated recombination (TAR) cloning for genomics studies and synthetic biology. Chromosoma 125, 621–632 (2016). https://doi.org/10.1007/s00412-016-0588-3

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  • DOI: https://doi.org/10.1007/s00412-016-0588-3

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